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Titolo:
Immunohistochemical distribution of RGS7 protein and cellular selectivity in colocalizing with G alpha q proteins in the adult rat brain
Autore:
Khawaja, XZ; Liang, JJ; Saugstad, JA; Jones, PG; Harnish, S; Conn, PJ; Cockett, MI;
Indirizzi:
Wyeth Ayerst Res, CNS Disorders, Princeton, NJ 08543 USA Wyeth Ayerst ResPrinceton NJ USA 08543 isorders, Princeton, NJ 08543 USA Emory Univ, Dept Pharmacol, Atlanta, GA 30322 USA Emory Univ Atlanta GA USA 30322 iv, Dept Pharmacol, Atlanta, GA 30322 USA
Titolo Testata:
JOURNAL OF NEUROCHEMISTRY
fascicolo: 1, volume: 72, anno: 1999,
pagine: 174 - 184
SICI:
0022-3042(199901)72:1<174:IDORPA>2.0.ZU;2-X
Fonte:
ISI
Lingua:
ENG
Soggetto:
GTPASE-ACTIVATING PROTEINS; PHOSPHOLIPASE-C; SUBUNITS; GAIP; EXPRESSION; KINASE; FAMILY; DOMAIN; PALMITOYLATION; REGULATORS;
Keywords:
regulator of G protein signaling 7; G alpha q/11; brain localization; immunohistochemistry; rat; confocal microscopy;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
36
Recensione:
Indirizzi per estratti:
Indirizzo: Khawaja, XZ Wyeth Ayerst Res, CNS Disorders, CN 8000, Princeton, NJ 08543 USA Wyeth Ayerst Res CN 8000 Princeton NJ USA 08543 , NJ 08543 USA
Citazione:
X.Z. Khawaja et al., "Immunohistochemical distribution of RGS7 protein and cellular selectivity in colocalizing with G alpha q proteins in the adult rat brain", J NEUROCHEM, 72(1), 1999, pp. 174-184

Abstract

Regulators of G protein signaling (RGS) proteins serve as potent GTPase-activating proteins for the heterotrimeric G proteins alpha i/o and alpha q/11. This study describes the immunohistochemical distribution of RGS7 throughout the adult rat brain and its cellular colocalization with G alpha q/11,an important G protein-coupled receptor signal transducer for phospholipase C beta-mediated activity. In general, both RGS7 and G alpha q/11 displayed a heterogeneous and overlapping regional distribution. RGS7 immunoreactivity was observed in cortical layers I-VI, being most intense in the neuropil of layer I. In the hippocampal formation, RGS7 immunoreactivity was concentrated in the strata oriens, strata radiatum, mossy fibers, and polymorphic cells, with faint to nondetectable immunolabeling within the dentate gyrus granule cells and CA1-CA3 subfield pyramidal cells. Numerous diencephalicand brainstem nuclei also displayed dense RGS7 immunostaining. Dual immunofluorescence labeling studies with the two protein-specific antibodies indicated a cellular selectivity in the colocalization between RGS7 and G alphaq/11 within many discrete brain regions, such as the superficial cortical layer I, hilus area of the hippocampal formation, and cerebellar Golgi cells. To assess the ability of G alpha q/11-mediated signaling pathways to modulate dynamically RGS expression, primary cortical neuronal cultures were incubated with phorbol 12,13-dibutyrate, a selective protein kinase C activator. A time-dependent increase in levels of mRNA for RGS7, but not RGS4, was observed. Our results provide novel information on the region- and cell-specific pattern of distribution of RGS7 with the transmembrane signal transducer, G alpha q/11. We also describe a possible RGS7-selective neuronal feedback adaptation on G alpha q/11-mediated pathway function, which may playan important role in signaling specificity in the brain.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 25/01/21 alle ore 19:23:54