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Titolo:
Detection of t(14;18) carrying cells in bone marrow and peripheral blood from patients affected by non-lymphoid diseases
Autore:
Rauzy, O; Galoin, S; Chale, JJ; Adoue, D; Albarede, JL; Delsol, G; Al Saati, T;
Indirizzi:
CHU Purpan, CNRS, ERS 1590, Dept Pathol, F-31300 Toulouse, France CHU Purpan Toulouse France F-31300 Dept Pathol, F-31300 Toulouse, France CHUulouse,, CNRS, ERS 1590, Unite Physiopathol Cellulaire & Mol, F-31300 To CHU Purpan Toulouse France F-31300 iopathol Cellulaire & Mol, F-31300 To CHU Purpan, Dept Geriatr Med, F-31300 Toulouse, France CHU Purpan Toulouse France F-31300 Geriatr Med, F-31300 Toulouse, France Hotel Dieu, Dept Biostat, F-31000 Toulouse, France Hotel Dieu Toulouse France F-31000 ept Biostat, F-31000 Toulouse, France
Titolo Testata:
JOURNAL OF CLINICAL PATHOLOGY-MOLECULAR PATHOLOGY
fascicolo: 6, volume: 51, anno: 1998,
pagine: 333 - 338
SICI:
1366-8714(199812)51:6<333:DOTCCI>2.0.ZU;2-5
Fonte:
ISI
Lingua:
ENG
Soggetto:
POLYMERASE CHAIN-REACTION; MINIMAL RESIDUAL DISEASE; FOLLICULAR LYMPHOMA; ONCOGENE TRANSLOCATION; B-CELLS; AMPLIFICATION; SEQUENCE; REARRANGEMENTS; CLASSIFICATION; PROGRESSION;
Keywords:
t(14;18) (q32;q21) translocation; bone marrow; non-lymphoid diseases; semi-nested polymerase chain reaction;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Clinical Medicine
Life Sciences
Citazioni:
26
Recensione:
Indirizzi per estratti:
Indirizzo: Al Saati, T CHUuse,pan, CNRS, ERS 1590, Dept Pathol, Ave Grande Bretagne, F-31300 Toulo CHU Purpan Ave Grande Bretagne Toulouse France F-31300 Toulo
Citazione:
O. Rauzy et al., "Detection of t(14;18) carrying cells in bone marrow and peripheral blood from patients affected by non-lymphoid diseases", J CL PATH-M, 51(6), 1998, pp. 333-338

Abstract

Aims/Background-To assess the presence of bcl-2/J(H) rearrangements in bone marrow and peripheral blood lymphocytes from patients affected by diseases other than malignant lymphomas. The t(14;18) (q32;q21) translocation, which juxtaposes the bcl-2 oncogene on chromosome 18 and the J(H) segment of the immunoglobulin heavy chain (IgH) genes on chromosome 14, is found frequently in follicular lymphomas. Methods-A sensitive semi-nested polymerase chain reaction (PCR) was used to detect t(14;18) translocation in bone marrow aspirates and peripheral blood lymphocytes from 48 patients. In 137 additional individuals peripheral blood lymphocytes only were tested. Results-Cells carrying bcl-2/J(H) rearrangements were detected in about a quarter of the bone marrow samples and half of the peripheral blood lymphocyte samples. In seven patients, t(14;18) positive cells were found in both the bone marrow and peripheral blood lymphocyte samples. The size of the PCR products and bcl-2/J(H) DNA sequence analysis showed that the same t(14;18) carrying clone was present in the bone marrow and the corresponding peripheral blood lymphocyte samples in three of these seven patients. Some patients had more than one bcl-2/J(H) rearrangement. There was no significant correlation between age and the translocation incidence. Cells carrying the t(14;18) translocation were present in peripheral blood lymphocyte samples with a similar incidence-between 47% and 52% in all age groups from 20 to 79 years. Patients older than 80 years had a lower (37%) but not significantly different incidence. Conclusions-These findings suggest that patients affected by non-lymphoid diseases may have several t(14;18) carrying cells and some of them undergo a clonal expansion. Whether individuals with t(14;18) positive cells are ata higher risk of lymphoid malignancies remains unanswered and further epidemiological studies are required.

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Documento generato il 29/11/20 alle ore 00:07:53