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Titolo:
Detection of Pneumocystis carinii DNA in blood specimens from human immunodeficiency virus-infected patients by nested PCR
Autore:
Rabodonirina, M; Cotte, L; Boibieux, A; Kaiser, K; Mayencon, M; Raffenot, D; Trepo, C; Peyramond, D; Picot, S;
Indirizzi:
Univ Lyon 1, Dept Parasitol & Mycol, F-69373 Lyon 08, France Univ Lyon 1 Lyon France 08 pt Parasitol & Mycol, F-69373 Lyon 08, France Hop Hotel Dieu, Serv Hepatogastroenterol & Sidol, F-69288 Lyon, France HopHotel Dieu Lyon France F-69288 enterol & Sidol, F-69288 Lyon, France Hop Croix Rousse, Serv Malad Infect & Trop, F-69317 Lyon, France Hop CroixRousse Lyon France F-69317 Infect & Trop, F-69317 Lyon, France
Titolo Testata:
JOURNAL OF CLINICAL MICROBIOLOGY
fascicolo: 1, volume: 37, anno: 1999,
pagine: 127 - 131
SICI:
0095-1137(199901)37:1<127:DOPCDI>2.0.ZU;2-6
Fonte:
ISI
Lingua:
ENG
Soggetto:
POLYMERASE CHAIN-REACTION; AIDS PATIENTS; PNEUMONIA; SERUM; AMPLIFICATION; DIAGNOSIS; SEQUENCES; RAT;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Clinical Medicine
Life Sciences
Citazioni:
23
Recensione:
Indirizzi per estratti:
Indirizzo: Rabodonirina, M Univancen 1, Dept Parasitol & Mycol, 8 Ave Rockefeller, F-69373 Lyon 08, Fr Univ Lyon 1 8 Ave Rockefeller Lyon France 08 Lyon 08, Fr
Citazione:
M. Rabodonirina et al., "Detection of Pneumocystis carinii DNA in blood specimens from human immunodeficiency virus-infected patients by nested PCR", J CLIN MICR, 37(1), 1999, pp. 127-131

Abstract

The detection of Pneumocystis carinii DNA in blood by PCR could be useful for studying the natural history of pneumocystosis and could also be a noninvasive diagnostic method, The results of previous studies are neverthelessconflicting. In our study, we compared three commercially available DNA extraction kits (Gene-Releaser, QIAamp Tissue Kit, and ReadyAmp Genomic DNA Purification System) and proteinase K and proteinase K-phenol-chloroform treatments for the extraction of P, carinii DNA from dilutions of a P, cariniif, sp. hominis cyst suspension mixed with human whole blood. A rapid and simple nested PCR protocol which amplifies a portion of the mitochondrial large-subunit rRNA gene was applied to all the extraction products, The QIAmpTissue Kit was the most effective kit for the isolation of amplification-ready P, carinii DNA and was used with nested PCR for the testing of whole-blood specimens from 35 immunocompetent control patients and 84 human immunodeficiency virus (HIV)-infected patients investigated for pulmonary diseaseand/or fever. In HIV-infected patients, P, carinii DNA was detected by nested PCR in blood samples from 3 of 14 patients with microscopically proven P, carinii pneumonia, 7 of 22 patients who were considered to be colonized with P, carinii, and 9 of 48 patients who were neither infected nor colonized with P, carinii, P, carinii DNA was not detected in blood specimens fromthe 35 immunocompetent patients. P, carinii DNA in blood might represent viable P, carinii organisms or DNA complexes released from pulmonary phagocytes, In conclusion, P, carinii DNA may be detected in whole blood from HIV-infected patients, but the nature and the meaning of the circulating form of P, carinii remain to be established.

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Documento generato il 21/09/20 alle ore 06:31:21