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Titolo:
The recycling pathway of protein ERGIC-53 and dynamics of the ER-Golgi intermediate compartment
Autore:
Klumperman, J; Schweizer, A; Clausen, H; Tang, BL; Hong, WJ; Oorschot, V; Hauri, HP;
Indirizzi:
Univ Basel, Bioctr, Dept Pharmacol, CH-4056 Basel, Switzerland Univ BaselBasel Switzerland CH-4056 armacol, CH-4056 Basel, Switzerland Univ Utrecht, Sch Med, Dept Cell Biol, NL-3584 CX Utrecht, Netherlands Univ Utrecht Utrecht Netherlands NL-3584 CX 3584 CX Utrecht, Netherlands Fac Hlth Sci, Sch Dent, DK-2200 Copenhagen, Denmark Fac Hlth Sci Copenhagen Denmark DK-2200 ent, DK-2200 Copenhagen, Denmark Natl Univ Singapore, Inst Mol & Cell Biol, Singapore 0511, Singapore Natl Univ Singapore Singapore Singapore 0511 , Singapore 0511, Singapore
Titolo Testata:
JOURNAL OF CELL SCIENCE
, volume: 111, anno: 1998,
parte:, 22
pagine: 3411 - 3425
SICI:
0021-9533(199811)111:<3411:TRPOPE>2.0.ZU;2-8
Fonte:
ISI
Lingua:
ENG
Soggetto:
MAMMALIAN KDEL RECEPTOR; EARLY SECRETORY PATHWAY; GTP-BINDING-PROTEINS; ENDOPLASMIC-RETICULUM; BREFELDIN-A; CIS-GOLGI; MEMBRANE-PROTEIN; BETA-COP; SUBCELLULAR-LOCALIZATION; LIVING CELLS;
Keywords:
ER-Golgi intermediate compartment; ERGIC-53; beta-COP; immunoelectron microscopy; protein recycling;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
91
Recensione:
Indirizzi per estratti:
Indirizzo: Hauri, HP Univ Basel, Bioctr, Dept Pharmacol, CH-4056 Basel, Switzerland Univ Basel Basel Switzerland CH-4056 H-4056 Basel, Switzerland
Citazione:
J. Klumperman et al., "The recycling pathway of protein ERGIC-53 and dynamics of the ER-Golgi intermediate compartment", J CELL SCI, 111, 1998, pp. 3411-3425

Abstract

To establish recycling routes in the early secretory pathway we have studied the recycling of the ER-Golgi intermediate compartment (ERGIC) marker ERGIC-53 in HepG2 cells. Immunofluorescence microscopy showed progressive concentration of ERGIC-53 in the Golgi area at 15 degrees C. Upon rewarming to37 degrees C ERGIC-53 redistributed into the cell periphery often via tubular processes that largely excluded anterograde transported albumin. Immunogold labeling of cells cultured at 37 degrees C revealed ERGIC-53 predominantly in characteristic P-COP-positive tubulo-vesicular clusters both near the Golgi apparatus and in the cell periphery. Concentration of ERGIC-53 at 15 degrees C resulted from both accumulation of ERGIC-53 in the ERGIC and movement of ERGIC membranes closer to the Golgi apparatus. Upon rewarming to37 degrees C the labeling of ERGIC-53 in the ERGIC rapidly returned to normal levels whereas ERGIC-53's labeling in the cis-Golgi was unchanged. Temperature manipulations had no effect on the average number of ERGIC-53 clusters. Density gradient centrifugation indicated that the surplus ERGIC-53 accumulating in the ERGIC at 15 degrees C was rapidly transported to the ER upon rewarming. These results suggest that the ERGIC is a dynamic membrane system composed of a constant average number of clusters and that the major recycling pathway of ERGIC-53 bypasses the Golgi apparatus.

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Documento generato il 27/09/20 alle ore 07:14:44