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Titolo:
A WAT1 MUTANT OF FISSION YEAST IS DEFECTIVE IN CELL MORPHOLOGY
Autore:
KEMP JT; BALASUBRAMANIAN MK; GOULD KL;
Indirizzi:
VANDERBILT UNIV,HOWARD HUGHES MED INST NASHVILLE TN 37232 VANDERBILT UNIV,HOWARD HUGHES MED INST NASHVILLE TN 37232 VANDERBILT UNIV,DEPT CELL BIOL NASHVILLE TN 37232
Titolo Testata:
MGG. Molecular & general genetics
fascicolo: 2, volume: 254, anno: 1997,
pagine: 127 - 138
SICI:
0026-8925(1997)254:2<127:AWMOFY>2.0.ZU;2-A
Fonte:
ISI
Lingua:
ENG
Soggetto:
SCHIZOSACCHAROMYCES-POMBE; PROTEIN-KINASE; S-POMBE; SEPTUM FORMATION; SACCHAROMYCES-CEREVISIAE; GENETIC-ANALYSIS; GROWTH POLARITY; DIVISION CYCLE; F-ACTIN; CYTOKINESIS;
Keywords:
FISSION YEAST; ACTIN; WD-40 REPEATS; PROFILIN; CELL POLARITY;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Science Citation Index Expanded
Citazioni:
36
Recensione:
Indirizzi per estratti:
Citazione:
J.T. Kemp et al., "A WAT1 MUTANT OF FISSION YEAST IS DEFECTIVE IN CELL MORPHOLOGY", MGG. Molecular & general genetics, 254(2), 1997, pp. 127-138

Abstract

The organization of the actin cytoskeleton plays an integral role in cell morphogenesis of all eukaryotes. We have isolated a temperature-sensitive mutant in Schizosaccharomyces pombe, wat1-l, in which acting patches are delocalized, resulting in an elliptically shaped cell phenotype. Molecular cloning and DNA sequencing of wat1(+) showed that thegene encodes a 314 residue protein containing WD-40 repeats. Cells lacking wat1(+) are slow growing but viable at 25 degrees C and temperature-sensitive for growth above 33 degrees C. At restrictive temperature, wat1-d strains are phenotypically indistinguishable from wat1-l. When combined with a deletion for the wat1(+) gene, cdc mutants failed to elongate at restrictive temperature and exhibited alterations in actin patch localization. This analysis suggests that wat1(+) is requireddirectly or indirectly for polarized cell growth in S. pombe. Wat1p and a functional, epitope-tagged, version of Wat1p can be overproduced without inducing alterations in cell morphology.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 04/04/20 alle ore 15:00:17