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Titolo:
The effect of intermittent injections of CCK-8S and the CCK-A receptor antagonist devazepide on cell proliferation in exocrine rat pancreas
Autore:
Ohlsson, B; Borg, K; Rehfeld, JF; Ihse, I; Axelson, J;
Indirizzi:
Univ Lund Hosp, Dept Surg, S-22185 Lund, Sweden Univ Lund Hosp Lund Sweden S-22185 Hosp, Dept Surg, S-22185 Lund, Sweden Rigshosp, Dept Clin Biochem, Copenhagen, Denmark Rigshosp Copenhagen Denmark osp, Dept Clin Biochem, Copenhagen, Denmark
Titolo Testata:
INTERNATIONAL JOURNAL OF PANCREATOLOGY
fascicolo: 3, volume: 24, anno: 1998,
pagine: 211 - 218
SICI:
0169-4197(199812)24:3<211:TEOIIO>2.0.ZU;2-Q
Fonte:
ISI
Lingua:
ENG
Soggetto:
HIGH-FAT DIET; CHOLECYSTOKININ ANTAGONIST; TUMOR INITIATION; TIME-COURSE; GROWTH; CERULEIN; CANCER; L-364,718; BLOCKADE; L364,718;
Keywords:
cell proliferation; cholecystokinin; intermittent injections; pancreas;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
21
Recensione:
Indirizzi per estratti:
Indirizzo: Axelson, J Univ Lund Hosp, Dept Surg, S-22185 Lund, Sweden Univ Lund HospLund Sweden S-22185 Surg, S-22185 Lund, Sweden
Citazione:
B. Ohlsson et al., "The effect of intermittent injections of CCK-8S and the CCK-A receptor antagonist devazepide on cell proliferation in exocrine rat pancreas", INT J PANCR, 24(3), 1998, pp. 211-218

Abstract

Conclusion. Intermittent injections of sulfated cholecystokinin-8 (CCK-8S)or devazepide caused longlasting effects on cell proliferation in exocrinepancreas in contrast to continuous infusion. The acinar cells responded toboth treatments with changes in the labeling index (LI) during the whole study period. When studying the influence of stimulation and inhibition of the CCK-A receptor on cell proliferation in the exocrine pancreas, not only are the drugs and doses of importance but also the mode of administration. Background. Continuous infusion of CCK-8S or the CCK-A receptor antagonistdevazepide induces transient changes in acinar cell proliferation in rat pancreas. The aim of the present experiments was to study whether intermittent administration of CCK-8S or devazepide prevents receptor desensitizationand thereby affects exocrine pancreatic cell proliferation persistently. Methods. Male Sprague-Dawley rats were injected subcutaneously twice dailywith CCK-8S (6 mu g), devazepide (240 mu g) or bovine serum albumin (BSA). The rats were sacrificed after 18 and 36 h and 3 and 7 d. One hour before sacrifice, the rats were injected intraperitoneally with 1 mCi/kg of tritiated thymidine, The pancreatic weight and the contents of water, protein, and DNA were determined. The LI (number of labeled cells/100 cells) of exocrine pancreatic cells was determined microscopically after autoradiography. Results. The concentration of plasma CCK was slightly increased by devazepide, but the increase was more pronounced by CCK-8S. The pancreatic wet weight was transiently increased 18 h after the start of CCK-8S injections (+14%), whereas devazepide caused a reduction after 7 d (-22%). The protein content was uninfluenced and the DNA content was decreased at 36 h with either treatment. CCK-8S increased the LI in acinar and centroacinar cells throughout the study period, but the ductal cell LI was increased only after 18 and 3 6 h. Injection of devazepide was followed by decreased LI of acinar cells throughout the study period. Also, the centroacinar and ductal cell LIdecreased initially but returned to control values after 7 d.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 22/10/20 alle ore 03:07:11