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Titolo:
CDNA CLONING AND EXPRESSION OF POKEWEED ANTIVIRAL PROTEIN FROM SEEDS IN ESCHERICHIA-COLI AND ITS INHIBITION OF PROTEIN-SYNTHESIS IN-VITRO
Autore:
POYET JL; HOEVELER A;
Indirizzi:
UFR SCI & TECH,LAB BIOCHIM & BIOL MOL,16 ROUTE GRAY F-25030 BESANCON FRANCE UFR SCI & TECH,LAB BIOCHIM & BIOL MOL F-25030 BESANCON FRANCE
Titolo Testata:
FEBS letters
fascicolo: 1-2, volume: 406, anno: 1997,
pagine: 97 - 100
SICI:
0014-5793(1997)406:1-2<97:CCAEOP>2.0.ZU;2-0
Fonte:
ISI
Lingua:
ENG
Soggetto:
RIBOSOME-INACTIVATING PROTEINS; PHYTOLACCA-AMERICANA; EUKARYOTIC RIBOSOMES; SEQUENCE; PURIFICATION; PLANTS; VIRUS; BINDING; CELLS; SITE;
Keywords:
CDNA; RIBOSOME-INACTIVATING PROTEIN; PHYTOLACCA AMERICANA; POKEWEED ANTIVIRAL PROTEIN FROM SEEDS; EXPRESSION IN ESCHERICHIA-COLI; INHIBITION OF IN VITRO PROTEIN SYNTHESIS;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Science Citation Index Expanded
Citazioni:
28
Recensione:
Indirizzi per estratti:
Citazione:
J.L. Poyet e A. Hoeveler, "CDNA CLONING AND EXPRESSION OF POKEWEED ANTIVIRAL PROTEIN FROM SEEDS IN ESCHERICHIA-COLI AND ITS INHIBITION OF PROTEIN-SYNTHESIS IN-VITRO", FEBS letters, 406(1-2), 1997, pp. 97-100

Abstract

Pokeweed antiviral proteins (PAP) represent a family of protein toxins isolated from various organs and at different stages of development of Phytolacca americana (pokeweed). We isolated, sequenced and characterized for the first time a complete cDNA encoding a pokeweed antiviral protein expressed in seeds, The cDNA of PAP-S consists of 1249 nucleotides and encodes a mature 262 amino acid protein. Its predicted amino acid sequence is more similar to PAP (76%) than to PAP II (31%), It is known from literature that PAP-S is more active in inhibiting protein synthesis than other members of the PAP family. Therefore, the cDNAof PAP-S was expressed in Escherichia coli and the biological activity of the recombinant protein was compared with that of PAP purified from spring leaves, In a rabbit translation system, the median inhibitory concentrations (IC50) Of recombinant PAP-S and native PAP were determined as 0.07 and 0.29 nM, respectively, Although the PAP-S protein inseeds is glycosylated, PAP-S can be expressed in Escherichia coli in a very active form, indicating that posttranslational modification in pokeweed does not seem to alter its ability to inhibit protein synthesis. (C) 1997 Federation of European Biochemical Societies.

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Documento generato il 01/04/20 alle ore 11:36:45