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Titolo:
Neonatal estrogen stimulates proliferation of periductal fibroblasts and alters the extracellular matrix composition in the rat prostate
Autore:
Chang, WY; Wilson, MJ; Birch, L; Prins, GS;
Indirizzi:
Univ Illinois, Dept Urol, Coll Med, Chicago, IL 60612 USA Univ Illinois Chicago IL USA 60612 Urol, Coll Med, Chicago, IL 60612 USA Univ Illinois, Dept Physiol & Biophys, Coll Med, Chicago, IL 60612 USA Univ Illinois Chicago IL USA 60612 ophys, Coll Med, Chicago, IL 60612 USA Univ Minnesota, Dept Lab Med, Minneapolis, MN 55417 USA Univ Minnesota Minneapolis MN USA 55417 ab Med, Minneapolis, MN 55417 USA Univ Minnesota, Dept Pathol, Minneapolis, MN 55417 USA Univ Minnesota Minneapolis MN USA 55417 Pathol, Minneapolis, MN 55417 USA Univ Minnesota, Dept Surg Oncol, Minneapolis, MN 55417 USA Univ MinnesotaMinneapolis MN USA 55417 Oncol, Minneapolis, MN 55417 USA VA Med Ctr, Minneapolis, MN 55417 USA VA Med Ctr Minneapolis MN USA 55417VA Med Ctr, Minneapolis, MN 55417 USA
Titolo Testata:
ENDOCRINOLOGY
fascicolo: 1, volume: 140, anno: 1999,
pagine: 405 - 415
SICI:
0013-7227(199901)140:1<405:NESPOP>2.0.ZU;2-J
Fonte:
ISI
Lingua:
ENG
Soggetto:
ANDROGEN RECEPTOR EXPRESSION; ADULT-RAT; PLASMINOGEN-ACTIVATOR; NEOPLASTIC PROSTATE; CELL-PROLIFERATION; VENTRAL PROSTATE; EXPOSURE; GROWTH; GLAND; LOBES;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
40
Recensione:
Indirizzi per estratti:
Indirizzo: Prins, GS Univ2Illinois, Dept Urol, Coll Med, M-C 955,820 S Wood St, Chicago, IL 6061 Univ Illinois M-C 955,820 S Wood St Chicago IL USA 60612 IL 6061
Citazione:
W.Y. Chang et al., "Neonatal estrogen stimulates proliferation of periductal fibroblasts and alters the extracellular matrix composition in the rat prostate", ENDOCRINOL, 140(1), 1999, pp. 405-415

Abstract

The purpose of this study was to examine whether changes in extracellular matrix (ECM) molecules are associated with the growth inhibition and differentiation defects of the prostate gland following neonatal exposure to estradiol. Using immunocytochemistry (ICC), laminin and collagen IV were localized to the basement membrane (BM) as well to the basal lamina of the periductal smooth muscle of the control developing prostates. In contrast, fibronectin and collagen III were localized throughout the stromal ECM. Exposure to neonatal estrogen altered the staining profile for specific ECM molecules. In the estrogenized rats, a thick layer of cells negative for laminin and collagen IV was observed adjacent to the BM. Electron microscopy and ICC for alpha-actin, fibronectin, and vimentin identified this multicellular layer of periductal cells as differentiated fibroblasts. Peripheral to these fibroblasts, actin-positive smooth muscle formed a second layer of periductal stromal cells. PCNA labeling showed that estrogen exposure increased thefibroblast proliferation. Because many periductal fibroblasts were positive for estrogen receptor alpha (ER alpha) in estrogenized rats, a direct effect of estradiol on their proliferation is suggested. Gelatinolytic gels revealed that estrogen exposure did not alter the activity of matrix metalloproteinases associated with tissue remodeling during prostate morphogenesis. However, the periductal fibroblast layer in estrogenized prostates was devoid of urokinase- and tissue-plasminogen activator, which may potentially alter the localized proteolysis involved in matrix remodeling. It is proposed that proliferation of a multicellular layer of periductal fibroblasts in estrogenized prostates results in a physical barrier that constrains branching morphogenesis and blocks paracrine communications between smooth muscleand epithelial cells which normally regulate differentiation.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 29/03/20 alle ore 15:11:47