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Titolo:
Regulation of IL-17, IFN-gamma and IL-10 in human CD8(+) T cells by cyclicAMP-dependent signal transduction pathway
Autore:
Shin, HCK; Benbernou, N; Fekkar, H; Esnault, S; Guenounou, M;
Indirizzi:
Fac Pharm, Immunol Lab, F-51100 Reims, France Fac Pharm Reims France F-51100 Pharm, Immunol Lab, F-51100 Reims, France Univ Wisconsin, Dept Pathol & Lab Med, Madison, WI 53792 USA Univ Wisconsin Madison WI USA 53792 thol & Lab Med, Madison, WI 53792 USA
Titolo Testata:
CYTOKINE
fascicolo: 11, volume: 10, anno: 1998,
pagine: 841 - 850
SICI:
1043-4666(199811)10:11<841:ROIIAI>2.0.ZU;2-M
Fonte:
ISI
Lingua:
ENG
Soggetto:
HUMAN-IMMUNODEFICIENCY-VIRUS; BLOOD MONONUCLEAR-CELLS; DIFFERENTIAL REGULATION; CYTOKINE PRODUCTION; MESSENGER-RNA; INFLAMMATORY RESPONSES; LYMPHOKINE PRODUCTION; INTERFERON-GAMMA; T(H)2 CYTOKINES; ATOPIC PATIENTS;
Keywords:
cAMP; CD8(+) T lymphocytes; IL-10; IL-17; IFN-gamma;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
42
Recensione:
Indirizzi per estratti:
Indirizzo: Benbernou, N Fac Pharm, Immunol Lab, 51 Rue Cognacq Jay, F-51100 Reims, France Fac Pharm 51 Rue Cognacq Jay Reims France F-51100 ms, France
Citazione:
H.C.K. Shin et al., "Regulation of IL-17, IFN-gamma and IL-10 in human CD8(+) T cells by cyclicAMP-dependent signal transduction pathway", CYTOKINE, 10(11), 1998, pp. 841-850

Abstract

In the present study, the expression of interleukin 17 (IL-17) by human CD8(+) T lymphocytes and its regulation following PKA activation was determined and compared with that of interferon gamma (IFN-gamma) and IL-10. IL-17 mRNA. was highly expressed in human CD8(+) T lymphocytes at least at the same level than in CD4(+) T cells that were isolated from peripheral blood. mononuclear cells (PBMC). Expression of IL-17 mRNA in CD8(+) T cells was induced by prior activation of PBMC for 18 h with Ca2+ ionophore and phorbol myristate acetate (PMA), Furthermore, our results clearly showed that CD8(+)T cells are sensitive to elevation of cAMP and PKA activation pathway. Data demonstrated a significant inhibition of IL-17 as well as of IFN-gamma mRNA expression in CD8(+) T cells isolated from activated PBMC cultured in the presence of either dibutyryl cAMP (db-cAMP) or PGE(2), In contrast, IL-10mRNA expression was strongly enhanced in the same experimental conditions. The differential expression of IL-10 and IFN-gamma production in CD8(+) T cells was aslo observed at the protein level as it was measured by a doubleimmunofluorescence technique and bow cytometry analysis. Taken together, these results provide evidence that human CD8(+) T cells are also the sourceof massive expression of IL-17, and that PKA plays a prominent role in theswitch of CD8(+)T cells to a Th2 like profile and an inhibition of IL-17 expression, thus suggesting that the activation of cAMP signal transduction pathway may have consequences for the relative role of CD8(+) T cells in the immune and inflammatory processes. (C) 1998 Academic Press.

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Documento generato il 14/07/20 alle ore 09:16:10