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Titolo:
Identification of phospholipase C beta isoforms and their location in cultured vascular smooth muscle cells of pig, human and rat
Autore:
Blayney, L; Gapper, P; Rix, C;
Indirizzi:
Univ Wales Coll Med, Dept Cardiol, Cardiff CF4 4XN, S Glam, Wales Univ Wales Coll Med Cardiff S Glam Wales CF4 4XN f CF4 4XN, S Glam, Wales
Titolo Testata:
CARDIOVASCULAR RESEARCH
fascicolo: 3, volume: 40, anno: 1998,
pagine: 564 - 572
SICI:
0008-6363(199812)40:3<564:IOPCBI>2.0.ZU;2-6
Fonte:
ISI
Lingua:
ENG
Soggetto:
HETEROTRIMERIC G-PROTEINS; SWISS 3T3 CELLS; SIGNAL-TRANSDUCTION; GAMMA-SUBUNITS; DIFFERENTIATION; PROLIFERATION; TRANSLOCATION; LOCALIZATION; ORGANIZATION; INHIBITION;
Keywords:
rat; pig; human; vascular smooth muscle cell cultures; phospholipase C; intracellular localisation; confocal microscopy;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Clinical Medicine
Life Sciences
Citazioni:
38
Recensione:
Indirizzi per estratti:
Indirizzo: Blayney, L Univ Wales Coll Med, Dept Cardiol, Hlth Pk, Cardiff CF4 4XN, S Glam, Wales Univ Wales Coll Med Hlth Pk Cardiff S Glam Wales CF4 4XN Wales
Citazione:
L. Blayney et al., "Identification of phospholipase C beta isoforms and their location in cultured vascular smooth muscle cells of pig, human and rat", CARDIO RES, 40(3), 1998, pp. 564-572

Abstract

Objectives: Four phospholipase C (PLC) beta isoforms have been described in pig aortic vascular smooth muscle. The aim was to determine if all four PLC beta isoforms are commonly expressed in vascular smooth muscle cells (VSMC) of three species, i.e. pig, human and rat, and if the individual isoforms had distinctive intracellular distributions. Methods: Vascular smooth muscle cell cultures were derived from explants of porcine and rat aorta and a human renal artery cell line. PLC beta isoform content was resolved usingWestern blotting. Intracellular location was determined by immunocytochemistry and confocal microscopy. Results: All three species expressed PLCs beta(1), beta(2), beta(3) and beta(4). In all species, PLC beta(1) demonstrated foci of concentration throughout the cytoplasm; PLC beta(2) demonstrated a punctate pattern that was principally at the cell periphery or was in theGolgi, depending upon the antibody used; PLC beta(3) was, also cytoplasmicbut showed a different pattern from PLC beta(1) and PLC beta(4) was cytoplasmic, except in pig quiescent cells, where it was associated with filamentous structures at the intersection with the plasma membrane. Conclusions: VSMCs of three different species express all four PLC beta isoforms. Each isoform has a unique and consistent signature of distribution that is generally common to all species. (C) 1998 Elsevier Science BN. All rights reserved.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 23/09/20 alle ore 21:48:42