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Titolo:
Chondrocyte-seeded hydroxyapatite for repair of large articular cartilage defects. A pilot study in the goat
Autore:
van Susante, JLC; Buma, P; Homminga, GN; van den Berg, WB; Veth, RPH;
Indirizzi:
UnivNetherlandsosp, Dept Orthoped, Orthopaed Res Lab, NL-6500 HB Nijmegen,Univ Nijmegen Hosp Nijmegen Netherlands NL-6500 HB NL-6500 HB Nijmegen, Priveklin Klein Rosendaal, Rozendaal, Netherlands Priveklin Klein Rosendaal Rozendaal Netherlands Rozendaal, Netherlands Univ Nijmegen Hosp, Lab Expt Rheumatol, NL-6500 HB Nijmegen, Netherlands Univ Nijmegen Hosp Nijmegen Netherlands NL-6500 HB Nijmegen, Netherlands
Titolo Testata:
BIOMATERIALS
fascicolo: 24, volume: 19, anno: 1998,
pagine: 2367 - 2374
SICI:
0142-9612(199812)19:24<2367:CHFROL>2.0.ZU;2-8
Fonte:
ISI
Lingua:
ENG
Soggetto:
MEDIAL FEMORAL CONDYLE; IN-VITRO; OSTEOCHONDRAL DEFECTS; CANINE CHONDROCYTES; COLLAGEN GEL; IMPLANTS; RABBIT; BONE; BEHAVIOR; KNEE;
Keywords:
cartilage repair; hydroxyapatite; chondrocyte; transplantation;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
33
Recensione:
Indirizzi per estratti:
Indirizzo: van Susante, JLC UnivNijmegen, Hosp, Dept Orthoped, Orthopaed Res Lab, POB9101, NL-6500 HB Univ Nijmegen Hosp POB 9101 Nijmegen Netherlands NL-6500HB
Citazione:
J.L.C. van Susante et al., "Chondrocyte-seeded hydroxyapatite for repair of large articular cartilage defects. A pilot study in the goat", BIOMATERIAL, 19(24), 1998, pp. 2367-2374

Abstract

The aim of this study was to evaluate the potential for restoration of a large cartilage defect in the goat knee with hydroxyapatite (HA) loaded withchondrocytes. Isolated chondrocytes were suspended in fibrin glue, seeded on top of the HA, and then the composite graft was implanted in the defect. After transplantation, cell behaviour, newly synthesised matrix and the HA-glue interface were assessed histologically after 2, 4, 12, 26 and 52 weeks. Special attention was paid to the incorporation process of HA in the subchondral bone and interactions between this biomaterial and the fibrin-glue-chondrocyte suspension. Chondrocytes in the glue proved to survive the transplantation procedure and produced new metachromatically stained matrix two weeks after implantation. The glue-cell suspension had penetrated the superficial porous structure of the HA. Four weeks after surgery, islands of hyaline-like cartilage were observed at the HA-glue interface. A layer of fibrous tissue was formed surrounding the HA graft, resulting in a relatively instable fixation of the HA in the defect. This instability of the graft in the defect, possibly together with early weight bearing, resulted in a gradual loss of the newly formed hyaline cartilage-like repair tissue. Progressive resorption of the HA occurred without any sign of active bone remodelling from the host site. One year after surgery part of the defect which extended down to the cancellous bone had been predominantly restored with newly formed lamellar bone. Only small HA remnants were still present at the bottom of the original defect. Resurfacing of the joint had occurred with fibrocartilaginous repair tissue. The absence of adequate fixation capacity of the Hd near the joint space resulted in a relative instability of the graft with progressive resorption. Therefore, HA is not a suitable biomaterial to facilitate the repair of large articular cartilage defects. (C) 1998 Elsevier Science Ltd. All rights reserved.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 13/07/20 alle ore 04:21:04