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Titolo:
Characteristics and regulation of the expression of interleukin 1 receptors by murine Langerhans cells and keratinocytes
Autore:
Cumberbatch, M; Dearman, RJ; Kimber, I;
Indirizzi:
Zeneca Cent Toxicol Lab, Macclesfield SK10 4TJ, Cheshire, England Zeneca Cent Toxicol Lab Macclesfield Cheshire England SK10 4TJ e, England
Titolo Testata:
ARCHIVES OF DERMATOLOGICAL RESEARCH
fascicolo: 12, volume: 290, anno: 1998,
pagine: 688 - 695
SICI:
0340-3696(199812)290:12<688:CAROTE>2.0.ZU;2-Y
Fonte:
ISI
Lingua:
ENG
Soggetto:
NECROSIS-FACTOR-ALPHA; COLONY-STIMULATING FACTOR; DRAINING LYMPH-NODES; IL-1 SIGNAL TRANSDUCTION; DENDRITIC CELLS; II RECEPTOR; CONTACT HYPERSENSITIVITY; CYTOKINE RECEPTORS; T-CELLS; ANTAGONIST;
Keywords:
Langerhans cells; keratinocytes; dendritic cells; interleukin 1; receptors; contact sensitization;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
39
Recensione:
Indirizzi per estratti:
Indirizzo: Cumberbatch, M Zenecaandnt Toxicol Lab, Alderley Pk, Macclesfield SK10 4TJ, Cheshire, Engl Zeneca Cent Toxicol Lab Alderley Pk Macclesfield Cheshire England SK10 4TJ
Citazione:
M. Cumberbatch et al., "Characteristics and regulation of the expression of interleukin 1 receptors by murine Langerhans cells and keratinocytes", ARCH DERM R, 290(12), 1998, pp. 688-695

Abstract

There is increasing evidence that interleukin 1 beta (IL-1 beta), a product in murine epidermis of Langerhans cells (LC) exclusively, contributes to the initiation and regulation of LC migration in response to skin sensitization. The hypothesis is that IL-1 beta induces the production by keratinocytes of tumour necrosis factor alpha (TNF-alpha) which acts in a paracrine fashion on LC to provide one signal for migration. In addition, it is believed that IL-1 beta acts in an autocrine fashion on LC to provide a second, TNF-alpha-independent, signal for the initiation of this response. The viability of this hypothesis is dependent upon the availability of appropriate membrane receptors. We describe therefore experiments designed to investigate the IL-1 receptor (IL-1R) status of keratinocytes, LC and lymph node dendritic cells (DC). Flow cytometric analyses of epidermal cell suspensions revealed at least 60% of LC positive for the type I IL-1R (IL-1RI). In contrast, only a small proportion of keratinocytes displayed surface IL-1RI, although high intracellular expression of this receptor could be detected either by flow cytometric analysis of cells permeabilized with saponin or by immunofluorescence microscopy. Expression of the type II IL-1R (IL-1RII) was detected at relatively low levels on both LC and keratinocytes. Interestingly, DC isolated from the lymph nodes of sensitized mice displayed upregulated expression of IL-IRI and lower levels of IL-1RII compared to LC. The conclusion drawn is that the IL-1R phenotype of LC and keratinocytes under resting conditions is consistent with the proposed contribution of IL-1 beta toLC migration. Furthermore, the regulation of IL-1R expression by epidermalcells and DC will undoubtedly influence the development of cutaneous immune responses.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 25/11/20 alle ore 04:47:29