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Titolo:
Characterization of cosmids and telomeres in cytogenetic preparations by 3D confocal fluorescence
Autore:
Kahn, E; Philippe, C; Frouin, F; Di Paola, R; Bernheim, A;
Indirizzi:
CHU Pitie Salpetriere, INSERM U494, F-75634 Paris 13, France CHU Pitie Salpetriere Paris France 13 ERM U494, F-75634 Paris 13, France Inst Gustave Roussy, CNRS, URA 1967, Villejuif, France Inst Gustave Roussy Villejuif France CNRS, URA 1967, Villejuif, France
Titolo Testata:
ANALYTICAL AND QUANTITATIVE CYTOLOGY AND HISTOLOGY
fascicolo: 6, volume: 20, anno: 1998,
pagine: 477 - 482
SICI:
0884-6812(199812)20:6<477:COCATI>2.0.ZU;2-2
Fonte:
ISI
Lingua:
ENG
Soggetto:
SCANNING LASER MICROSCOPY; INSITU HYBRIDIZATION; CHROMOSOMES; CYTOMETRY; SEQUENCES; DEPTH;
Keywords:
computer-assisted image processing; in situ hybridization; fluorescence; confocal microscopy; chromosomes; cosmids; telomere;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
15
Recensione:
Indirizzi per estratti:
Indirizzo: Kahn, E CHU Pitie Salpetriere, INSERM U494, F-75634 Paris 13, France CHU Pitie Salpetriere Paris France 13 , F-75634 Paris 13, France
Citazione:
E. Kahn et al., "Characterization of cosmids and telomeres in cytogenetic preparations by 3D confocal fluorescence", ANAL QUAN C, 20(6), 1998, pp. 477-482

Abstract

OBJECTIVE: To analyze, with fluorescent probes, by three-dimensional (30) emission patterns, fluorescence in situ hybridization (FISH) preparations (cosmids, telomeres) and to perform factor analysis of medical image sequences (FAMIS); to use FISH to track relevant DNA sequences in cell nuclei during interphase and in mitotic chromosomes; and to use cytogenetic techniques, resulting in flat preparations of whole cells that are assumed to preserve probe access to their targets. STUDY DESIGN: The study design entailed labeling targets by probes (sequences labeled by fluorescein isothiocyanate) in nuclei and/or chromosomes stained by propidium iodine. Visualization of targets was improved when 30 sequences of images obtained on a single photomultiplier detector of the confocal microscope by z stepping were investigated by FAMIS. RESULTS: Factors and factor images showed that targets could be detected and differentiated in focal planes inside nuclei or chromosomes. CONCLUSION: It is possible to localize cosmids in cell n nuclei at interphase and telomeres in mitotic chromosomes by means of 30 sequences of images.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 04/04/20 alle ore 15:09:19