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Titolo:
Cleavage product from the NO donor NOC-5 and inner ear hair cell damage
Autore:
Takayama, M; Yamane, H; Konishi, K; Iguchi, H; Nakagawa, T; Shibata, S; Nishimura, K; Sunami, K; Nakai, Y;
Indirizzi:
Osaka City Univ, Sch Med, Dept Otolaryngol, Abeno Ku, Osaka 5458585, JapanOsaka City Univ Osaka Japan 5458585 ngol, Abeno Ku, Osaka 5458585, Japan
Titolo Testata:
ACTA OTO-LARYNGOLOGICA
, , anno: 1998, supplemento:, 538
pagine: 12 - 18
SICI:
0001-6489(1998):<12:CPFTND>2.0.ZU;2-L
Fonte:
ISI
Lingua:
ENG
Soggetto:
NITRIC-OXIDE; SODIUM-NITROPRUSSIDE; NEUROTOXICITY;
Keywords:
cochlea; guinea pig; necrosis;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Clinical Medicine
Life Sciences
Citazioni:
8
Recensione:
Indirizzi per estratti:
Indirizzo: Takayama, M Osakakaity Univ, Sch Med, Dept Otolaryngol, Abeno Ku, 1-5-7 Asahimachi, Osa Osaka City Univ 1-5-7 Asahimachi Osaka Japan 5458585 chi, Osa
Citazione:
M. Takayama et al., "Cleavage product from the NO donor NOC-5 and inner ear hair cell damage", ACT OTO-LAR, 1998, pp. 12-18

Abstract

Nitric oxide (NO) is a neurotoxic gas which causes neuronal cell death. NOototoxicity has also been reported. 1-Hydroxy-2-oxo-3-(3-aminopropyl)-3-isopropyl-1-triazene (NOC-5), a NO donor, was used for studying NO. In this in vitro study, we examined whether NOC-5 and the cleavage products arising from it after production of NO have ototoxic effects on the cochlea. The temporal bones of 55 guinea pigs were dissected and incubated in Dulbecco Modified Eagle's Medium (DMEM) with low glucose and 10%, foetal bovine serum (FBS) perfused with various concentrations (100, 50, 10, 1 mM, 100 mu M) of NOC-5 (pH 7.4) or the same concentrations of N-isopropyl 1-3 propanildiamine (R-NB2). Hair cell death was assessed using trypan blue staining. With NOC-5, percentages of outer hair cell death were 99.2, 98.0, 45.2, 40.5, and 20%, respectively; and percentages of inner hair cell death were 69.3, 70.2, 22.5. 11.1 and 0%, respectively. With R-NH2, percentages of outer hair cell death were 90.3, 81.1, 29.8, 21.0 and 19.3%, respectively, and percentages of inner hair cell death were 61.4, 48.1, 10.2, 5.4 and 0%, respectively. Ultramicroscopic examination showed that hair cell death was necrotic. Although NOC-5 is useful for study of NO, its cleavage products are also ototoxic.

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Documento generato il 29/11/20 alle ore 17:51:06