Catalogo Articoli (Spogli Riviste)

OPAC HELP

Titolo:
Assessment of hair cell death using the dye extrusion method
Autore:
Yamane, H; Konishi, K; Iguchi, H; Nakagawa, T; Shibata, S; Takayama, M; Nishimura, K; Sunami, K; Nakai, Y;
Indirizzi:
Osaka City Univ, Sch Med, Dept Otorhinolaryngol, Osaka 5458585, Japan Osaka City Univ Osaka Japan 5458585 orhinolaryngol, Osaka 5458585, Japan
Titolo Testata:
ACTA OTO-LARYNGOLOGICA
, , anno: 1998, supplemento:, 538
pagine: 7 - 11
SICI:
0001-6489(1998):<7:AOHCDU>2.0.ZU;2-F
Fonte:
ISI
Lingua:
ENG
Soggetto:
NITRIC-OXIDE;
Keywords:
inner ear; cochlea; trypan blue;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Clinical Medicine
Life Sciences
Citazioni:
6
Recensione:
Indirizzi per estratti:
Indirizzo: Yamane, H Osakakaity Univ, Sch Med, Dept Otorhinolaryngol, 1-5-7 AsahimachAbeno, Osa Osaka City Univ 1-5-7 Asahimach Abeno Osaka Japan 5458585 , Osa
Citazione:
H. Yamane et al., "Assessment of hair cell death using the dye extrusion method", ACT OTO-LAR, 1998, pp. 7-11

Abstract

We examined the usefulness of the dye extrusion method for assessing cochlear hair cell death in guinea pigs. Unilateral temporal bones were incubated in sterile Dulbecco modified Eagle's medium for 1-6 h and then perfused with PBS/0.3% trypan blur to determine hair cell viability. Cells with nuclei stained with trypan blue were assumed to have died. The contralateral temporal bones of each animal were incubated with the same medium for 1-6 h and examined by transmission electron microscopy (TEM). This method revealed that hair cell viability under these conditions was maintained for at most 3 h. After incubation for more than 3 ii, hair cell injury began and increased. After 6 h incubation, TEM revealed apoptotic death of outer hair cells. The results with this method agreed with the TEM findings. The dye extrusion method is simple, easy and useful for screening hair cell death in the cochlea under pathological conditions.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 02/12/20 alle ore 07:09:28