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Titolo:
Protection against lethal Japanese encephalitis virus infection of mice byimmunization with the highly attenuated MVA strain of vaccinia virus expressing JEV prM and E genes
Autore:
Nam, JH; Wyatt, LS; Chae, SL; Cho, HW; Park, YK; Moss, B;
Indirizzi:
Korean NIH, Dept Viral Dis, Div Arborviruses, Seoul, South Korea Korean NIH Seoul South Korea Dis, Div Arborviruses, Seoul, South Korea Korea Univ, Dept Biol, Seoul 136701, South Korea Korea Univ Seoul South Korea 136701 Dept Biol, Seoul 136701, South Korea NIAID, Viral Dis Lab, NIH, Bethesda, MD 20892 USA NIAID Bethesda MD USA 20892 D, Viral Dis Lab, NIH, Bethesda, MD 20892 USA
Titolo Testata:
VACCINE
fascicolo: 3, volume: 17, anno: 1999,
pagine: 261 - 268
SICI:
0264-410X(19990121)17:3<261:PALJEV>2.0.ZU;2-J
Fonte:
ISI
Lingua:
ENG
Soggetto:
HOST-RANGE; NUCLEOTIDE-SEQUENCE; RECOMBINANTS; IMMUNITY; PROTEIN; GENOME; GLYCOPROTEIN; INFLUENZA; SECRETION; VIREMIA;
Keywords:
Japanese encephalitis virus; vaccinia virus; MVA strain;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Agriculture,Biology & Environmental Sciences
Life Sciences
Citazioni:
30
Recensione:
Indirizzi per estratti:
Indirizzo: Moss, B 4 Ctr Dr MSC 0445, Bethesda, MD 20892 USA 4 Ctr Dr MSC 0445 Bethesda MD USA 20892 5, Bethesda, MD 20892 USA
Citazione:
J.H. Nam et al., "Protection against lethal Japanese encephalitis virus infection of mice byimmunization with the highly attenuated MVA strain of vaccinia virus expressing JEV prM and E genes", VACCINE, 17(3), 1999, pp. 261-268

Abstract

Genes encoding the glycosylated precursor of the membrane (prM) and envelope (E) proteins of a Korean strain of Japanese encephalitis virus (JEV) were inserted into the genome of the host-range restricted, highly attenuated,and safety-tested MVA strain of vaccinia virus. MVA recombinants containing the JEV genes, under strong synthetic or modified H5 vaccinia virus promoters, were isolated. Synthesis of JEV prM and E proteins was detected by immunofluorescence microscopy, flow cytometry, and polyacrylamide gel electrophoresis. Mice inoculated and boosted by various routes with either of the MVA recombinants produced JEV neutralizing antibodies, that had titres comparable with those induced by an inactivated JEV vaccine, as well as haemagglutination-inhibiting antibodies. Mice immunized with 2x10(6) infectious units of MVA/JEV recombinants by intramuscular or intraperitoneal routes werecompletely protected against a 10(5) LD50 JEV challenge at 9 weeks of age. (C) 1998 Elsevier Science Ltd. All rights reserved.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 10/04/20 alle ore 15:47:04