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Titolo:
Potentiation of the oxidative burst and isoflavonoid phytoalexin accumulation by serine protease inhibitors
Autore:
Guo, ZJ; Lamb, C; Dixon, RA;
Indirizzi:
Samuel Roberts Noble Fdn Inc, Div Plant Biol, Ardmore, OK 73401 USA SamuelRoberts Noble Fdn Inc Ardmore OK USA 73401 , Ardmore, OK 73401 USA Salk Inst Biol Studies, Plant Biol Lab, La Jolla, CA 92036 USA Salk Inst Biol Studies La Jolla CA USA 92036 Lab, La Jolla, CA 92036 USA
Titolo Testata:
PLANT PHYSIOLOGY
fascicolo: 4, volume: 118, anno: 1998,
pagine: 1487 - 1494
SICI:
0032-0889(199812)118:4<1487:POTOBA>2.0.ZU;2-X
Fonte:
ISI
Lingua:
ENG
Soggetto:
PLANT-DISEASE RESISTANCE; NADPH OXIDASE; SIGNAL-TRANSDUCTION; PLASMA-MEMBRANE; CELLS; ELICITATION; INVOLVEMENT; DEFENSE; GENE; ACTIVATION;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Agriculture,Biology & Environmental Sciences
Life Sciences
Citazioni:
35
Recensione:
Indirizzi per estratti:
Indirizzo: Dixon, RA Samuel Roberts Noble Fdn Inc, Div Plant Biol, 2510 Sam Noble Pkwy, Ardmore, Samuel Roberts Noble Fdn Inc 2510 Sam Noble Pkwy Ardmore OK USA73401
Citazione:
Z.J. Guo et al., "Potentiation of the oxidative burst and isoflavonoid phytoalexin accumulation by serine protease inhibitors", PLANT PHYSL, 118(4), 1998, pp. 1487-1494

Abstract

Treatment of soybean (Glycine max L. cv Williams 82) cell-suspension cultures with Pseudomonas syringae pv glycinea (Psg) harboring an avirulence gene (avrA) or with yeast elicitor resulted in an oxidative burst characterized by the accumulation of H2O2. This burst, and the resultant induction of glutathione S-transferase transcripts, occurred more rapidly and was more prolonged if cells were simultaneously treated with serine protease inhibitors such as phenylmethylsulfonyl fluoride (PMSF) or diisopropylfluorophosphate. PMSF and diisopropylfluorophosphate potentiate a large oxidative burst in cells exposed to Psg harboring the avrC avirulence gene, which is not recognized by the soybean cultivar used in this study. The potentiated burst was inhibited by diphenylene iodonium, an inhibitor of NADPH oxidase, and bythe protein kinase inhibitor K252a. PMSF treatment of elicited cells or cells exposed to Psg:avrA caused a large increase in the accumulation of the isoflavonoid phytoalexin glyceollin; however, this was not associated with increased levels of transcripts encoding key phytoalexin biosynthetic enzymes. Glyceollin accumulation was inhibited by diphenylene iodonium; however,the oxidative burst in cells treated with Psg:avrC and PMSF was not followed by phytoalexin accumulation. We conclude that active oxygen species fromthe oxidative burst are necessary but not sufficient for inducing isoflavonoid phytoalexin accumulation in soybean cells.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 28/09/20 alle ore 15:04:09