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Titolo:
Export of carbon from chloroplasts at night
Autore:
Schleucher, J; Vanderveer, PJ; Sharkey, TD;
Indirizzi:
Univ Wisconsin, Dept Bot, Madison, WI 53706 USA Univ Wisconsin Madison WIUSA 53706 nsin, Dept Bot, Madison, WI 53706 USA Univ Wisconsin, Dept Biochem, Madison, WI 53706 USA Univ Wisconsin Madison WI USA 53706 , Dept Biochem, Madison, WI 53706 USA
Titolo Testata:
PLANT PHYSIOLOGY
fascicolo: 4, volume: 118, anno: 1998,
pagine: 1439 - 1445
SICI:
0032-0889(199812)118:4<1439:EOCFCA>2.0.ZU;2-Q
Fonte:
ISI
Lingua:
ENG
Soggetto:
TRANSGENIC POTATO PLANTS; TRIOSE-PHOSPHATE TRANSLOCATOR; BARLEY PRIMARY LEAVES; PISUM-SATIVUM-L; SPINACH-CHLOROPLASTS; FRUCTOSE 2,6-BISPHOSPHATE; METABOLITE CONCENTRATIONS; ANTISENSE REPRESSION; SUBCELLULAR VOLUMES; STARCH;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Agriculture,Biology & Environmental Sciences
Life Sciences
Citazioni:
48
Recensione:
Indirizzi per estratti:
Indirizzo: Sharkey, TD Univ Wisconsin, Dept Bot, Madison, WI 53706 USA Univ Wisconsin Madison WI USA 53706 ot, Madison, WI 53706 USA
Citazione:
J. Schleucher et al., "Export of carbon from chloroplasts at night", PLANT PHYSL, 118(4), 1998, pp. 1439-1445

Abstract

Hexose export from chloroplasts at night has been inferred in previous studies of mutant and transgenic plants. We have tested whether hexose export is the normal route of carbon export from chloroplasts at night. We used nuclear magnetic resonance to distinguish glucose (Glc) made from hexose export and Glc made from triose export. Glc synthesized in vitro from fructose-6-phosphate in the presence of deuterium-labeled water had deuterium incorporated at C-2, whereas synthesis from triose phosphates caused C-2 through C-5 to become deuterated. In both tomato (Lycopersicon esculentum L.) and bean (Phaseolus vulgaris L.), Glc from sucrose made at night in the presenceof deuterium-enriched water was deuterated only in the C-2 position, indicating that >75% of carbon is exported as hexoses at night. In darkness the phosphate in the cytosol was 28 mM, whereas that in the chloroplasts was 5 mM, but hexose phosphates were 10-fold higher in the cytosol than in the chloroplasts. Therefore, hexose phosphates would not move out of chloroplastswithout the input of energy. We conclude that most carbon leaves chloroplasts at night as Glc, maltose, or higher maltodextrins under normal conditions.

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Documento generato il 01/04/20 alle ore 01:50:55