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Titolo:
Expression of mu, kappa, and delta opioid receptor messenger RNA in the human CNS: A P-33 in situ hybridization study
Autore:
Peckys, D; Landwehrmeyer, GB;
Indirizzi:
Univ Freiburg, Dept Neurol, Neurozentrum, D-79106 Freiburg, Germany Univ Freiburg Freiburg Germany D-79106 entrum, D-79106 Freiburg, Germany
Titolo Testata:
NEUROSCIENCE
fascicolo: 4, volume: 88, anno: 1999,
pagine: 1093 - 1135
SICI:
0306-4522(199902)88:4<1093:EOMKAD>2.0.ZU;2-G
Fonte:
ISI
Lingua:
ENG
Soggetto:
LONG-TERM POTENTIATION; OBSESSIVE-COMPULSIVE DISORDER; LUTEINIZING-HORMONE SECRETION; MORPHINE-INDUCED ANALGESIA; PITUITARY-ADRENAL AXIS; HUMAN CEREBRAL-CORTEX; RAT SUBSTANTIA-NIGRA; RHESUS-MONKEY BRAIN; GUINEA-PIG BRAIN; OPIATE RECEPTORS;
Keywords:
brain; human; in situ hybridization; messenger RNA; opioid receptors; phosphorus radioisotopes;
Tipo documento:
Review
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
204
Recensione:
Indirizzi per estratti:
Indirizzo: Landwehrmeyer, GB Univg,reiburg, Dept Neurol, Neurozentrum, Breisacherstr 64, D-79106 Freibur Univ Freiburg Breisacherstr 64 Freiburg Germany D-79106
Citazione:
D. Peckys e G.B. Landwehrmeyer, "Expression of mu, kappa, and delta opioid receptor messenger RNA in the human CNS: A P-33 in situ hybridization study", NEUROSCIENC, 88(4), 1999, pp. 1093-1135

Abstract

The existence of at least three opioid receptor types, referred to as mu, kappa, and delta, is well established. Complementary DNAs corresponding to the pharmacologically defined mu, kappa, and delta opioid receptors have been isolated in various species including man. The expression patterns of opioid receptor transcripts in human brain has not bean established with a cellular resolution, in part because of the low apparent abundance of opioid receptor messenger RNAs in human brain. To visualize opioid receptor messenger RNAs we developed a sensitive in situ hybridization histochemistry method using P-33-labelled RNA probes. In the present study we report the regional and cellular expression of mu, kappa, and delta opioid receptor messenger RNAs in selected areas of the human brain. Hybridization of the different opioid receptor probes resulted in distinct labelling patterns. For the mu and kappa opioid receptor probes, the most intense regional signals were observed in striatum, thalamus, hypothalamus, cerebral cortex, cerebellum and certain brainstem areas as well as the spinal cord. The most intense signals for the delta opioid receptor probe were found in cerebral cortex. Expression of opioid receptor transcripts was restricted to subpopulations of neurons within most regions studied demonstrating differences in the cellular expression patterns of mu, kappa, and delta opioid receptor messenger RNAs in numerous brain regions. The messenger RNA distribution patterns for each opioid receptor corresponded in general to the distribution of opioid receptor binding sites as visualized by receptor autoradiography. However, some mismatches, for instance between mu opioid receptor receptor binding and mu opioid receptor messenger RNA expression in the anterior striatum, were observed. A comparison of the distribution patterns of opioid receptor messenger RNAs in the human brain and that reported for the rat suggests a homologous expression pattern in many regions. However, in the human brain, kappa opioid receptor messenger RNA expression was more widely distributed than in rodents. The differential and region specific expression of opioid receptors may help to identify targets for receptor specific compounds in neuronal circuitsinvolved in a variety of physiological functions including pain perception, neuroendocrine regulation, motor control and reward. (C) 1998 IBRO. Published by Elsevier Science Ltd.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 02/04/20 alle ore 19:09:58