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Titolo:
Biological effect of helium-neon (He-Ne) laser irradiation on mouse myeloma (Sp2-Ag14) cell line in vitro
Autore:
Ocana-Quero, JM; de la Lastra, JP; Gomez-Villamandos, R; Moreno-Millan, M;
Indirizzi:
Fac Vet, Dept Genet, Cordoba 14005, Spain Fac Vet Cordoba Spain 14005Fac Vet, Dept Genet, Cordoba 14005, Spain Fac Vet, Dept Patol Anim, Cordoba 14005, Spain Fac Vet Cordoba Spain 14005 c Vet, Dept Patol Anim, Cordoba 14005, Spain
Titolo Testata:
LASERS IN MEDICAL SCIENCE
fascicolo: 3, volume: 13, anno: 1998,
pagine: 214 - 218
SICI:
0268-8921(1998)13:3<214:BEOH(L>2.0.ZU;2-G
Fonte:
ISI
Lingua:
ENG
Soggetto:
RADIATION; CULTURES; CYCLE;
Keywords:
cell proliferation; flow cytometry; He-Ne laser; photobiology; Sp2-Ag14 cell line;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Clinical Medicine
Life Sciences
Citazioni:
24
Recensione:
Indirizzi per estratti:
Indirizzo: Ocana-Quero, JM Fac Vet, Dept Genet, Cordoba 14005, Spain Fac Vet Cordoba Spain 14005 Genet, Cordoba 14005, Spain
Citazione:
J.M. Ocana-Quero et al., "Biological effect of helium-neon (He-Ne) laser irradiation on mouse myeloma (Sp2-Ag14) cell line in vitro", LASER MED S, 13(3), 1998, pp. 214-218

Abstract

We examined the effect of helium-neon (He-Ne) laser irradiation with a wavelength of 632.8 nm on cell cycle synchronisation of monolayer growing mouse myeloma (Sp2-Ag14) cell line. The monolayer cultures were exposed to repeated doses of different energy densities (4-64 J/cm(2)). The nuclear DNA content has been studied by flow cytometry to obtain the cell percentage in each cell cycle phase, Results showed that the He-Ne laser irradiation at energy densities of 8-64 J/cm(2) produced a significative and different effect on the G0-G1 and S phases of cell cycle over control. In contrast, no significant effect in G2-M phase was produced by He-Ne laser irradiation at any energy density compared with non-treated control. These results support previous observations suggesting that He-Ne laser irradiation of low energy density interferes with cell cycling and may inhibit cell proliferation when irradiation is performed at doses of 8 J/cm(2) or more.

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Documento generato il 26/11/20 alle ore 13:20:00