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Titolo:
Sequence-specific and/or stereospecific constraints of the U3 enhancer elements of MCF 247-W are important for pathogenicity
Autore:
DiFronzo, NL; Holland, CA;
Indirizzi:
Childrens20010 Med Ctr, Ctr Virol Immunol & Infect Dis Res, Washington, DCChildrens Natl Med Ctr Washington DC USA 20010 ct Dis Res, Washington, DC
Titolo Testata:
JOURNAL OF VIROLOGY
fascicolo: 1, volume: 73, anno: 1999,
pagine: 234 - 241
SICI:
0022-538X(199901)73:1<234:SASCOT>2.0.ZU;2-C
Fonte:
ISI
Lingua:
ENG
Soggetto:
MURINE LEUKEMIA-VIRUS; LONG TERMINAL REPEAT; I TRANSCRIPTIONAL ACTIVATORS; INDUCED THYMIC LYMPHOMAS; T-CELL LYMPHOMAS; C-MYC LOCUS; NONDEFECTIVE FRIEND; DISEASE SPECIFICITY; RETROVIRUS MCF-247; VIRAL SEQUENCES;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
59
Recensione:
Indirizzi per estratti:
Indirizzo: Holland, CA ChildrenseNatl Med Ctr, Ctr Virol Immunol & Infect Dis Res, 111 Michigan Av Childrens Natl Med Ctr 111 Michigan Ave NW Washington DC USA 20010
Citazione:
N.L. DiFronzo e C.A. Holland, "Sequence-specific and/or stereospecific constraints of the U3 enhancer elements of MCF 247-W are important for pathogenicity", J VIROLOGY, 73(1), 1999, pp. 234-241

Abstract

The oncogenic potential of many nonacute retroviruses is dependent on the duplication of the enhancer sequences present in the unique 3' (U3) region of the long terminal repeat (LTR). In a molecular clone (MCF 247-W) of the murine leukemia virus MCF 247, a leukemogenic mink cell focus-inducing (MCF) virus, the U3 enhancer sequences are tandemly repeated in the LTR. We mutated the enhancer region of MCF 247-W to test the hypothesis that the duplicated enhancer sequences of this virus have a sequence-specific and/or a ste reospecific role in enhancer function required for transformation. In onevirus, we inserted 14 nucleotide bp into the novel sequence generated at the junction of the two enhancers to generate an MCF virus with an interrupted enhancer region. In the second virus, only one copy of the enhancer sequences was present. This second virus also lacked the junction sequence present between the two enhancers of MCF 247-W. Both viruses were less leukemogenic and had a longer mean latency period than MCF 247-W. These data indicate that the sequence generated at the junction of the two enhancers and/or the stereospecific arrangement of the two enhancer elements are required for the full oncogenic potential of MCF 247-W. We analyzed proviral LTRs within the c-myc locus in tumor DNAs from mice injected with the MCF virus,viththe interrupted enhancer region. Some of the proviral LTRs integrated upstream of c-myc contain enhancer regions that are larger than those of the injected virus. These results are consistent with the suggestion that the virus with an interrupted enhancer changes in vivo to perform its role in the transformation of T cells.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 17/01/21 alle ore 18:09:07