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Titolo:
PCR sequencing of the spike genes of geographically and chronologically distinct human coronaviruses 229E
Autore:
Hays, JP; Myint, SH;
Indirizzi:
Univ Leicester, Dept Microbiol & Immunol, Leicester LE1 9HN, Leics, England Univ Leicester Leicester Leics England LE1 9HN er LE1 9HN, Leics, England Univlandcester, Prot & Nucleic Acid Chem Lab, Leicester LE1 9HN, Leics, Eng Univ Leicester Leicester Leics England LE1 9HN cester LE1 9HN, Leics, Eng
Titolo Testata:
JOURNAL OF VIROLOGICAL METHODS
fascicolo: 2, volume: 75, anno: 1998,
pagine: 179 - 193
SICI:
0166-0934(199811)75:2<179:PSOTSG>2.0.ZU;2-3
Fonte:
ISI
Lingua:
ENG
Soggetto:
RESPIRATORY CORONAVIRUS; NUCLEOTIDE-SEQUENCE; CANINE CORONAVIRUS; RNA RECOMBINATION; TOUCHDOWN PCR; CELL-FUSION; EXPRESSION; ANTIBODIES; GASTROENTERITIS; GLYCOPROTEIN;
Keywords:
PCR; human coronaviruses 229E; spike genes;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
35
Recensione:
Indirizzi per estratti:
Indirizzo: Myint, SH Univ Leicester, Dept Microbiol & Immunol, Leicester LE1 9HN, Leics, England Univ Leicester Leicester Leics England LE1 9HN , Leics, England
Citazione:
J.P. Hays e S.H. Myint, "PCR sequencing of the spike genes of geographically and chronologically distinct human coronaviruses 229E", J VIROL MET, 75(2), 1998, pp. 179-193

Abstract

A reverse transcription nested PCR (RT-PCR) sequencing methodology was developed and used to generate sequence data from the spike genes of three geographically and chronologically distinct human coronaviruses 229E. These three coronaviruses were isolated originally from the USA in the 1960s (humancoronavirus 229E strain ATCC VR-74), the UK in the 1990s (human coronavirus 229E LRI 281) and Ghana (human coronavirus 229E A162). Upon translation and alignment with the published spike protein sequence of human coronavirus229E 'LP' (isolated in the UI( in the 1970s), it was found that variation within the translated protein sequences was rather limited. In particular, minimal variation was observed between the translated spike protein sequence of human coronaviruses 229E LP and ATCC VR-74 (1/1012 amino acid differences), whilst most variation was observed between the translated spike protein sequence of human coronaviruses 229E LP and A162 (47/1012 amino acid changes). Further, the translated spike protein sequence of human coronavirus 229E A162 showed three clusters of amino acid changes, situated within the 5' half of the translated spike protein sequence. (C) 1998 Published by Elsevier Science B.V. All rights reserved.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 04/12/20 alle ore 19:24:59