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Titolo:
Yeast RNase III as a key processing enzyme in small nucleolar RNAs metabolism
Autore:
Chanfreau, G; Legrain, P; Jacquier, A;
Indirizzi:
Inst15,steur, Lab Metab ARN, CNRS, URA 1300,Dept Biotechnol, F-75724 ParisInst Pasteur Paris France 15 RS, URA 1300,Dept Biotechnol, F-75724 Paris
Titolo Testata:
JOURNAL OF MOLECULAR BIOLOGY
fascicolo: 4, volume: 284, anno: 1998,
pagine: 975 - 988
SICI:
0022-2836(199812)284:4<975:YRIAAK>2.0.ZU;2-S
Fonte:
ISI
Lingua:
ENG
Soggetto:
18S RIBOSOMAL-RNA; MOUSE U14 SNRNA; SACCHAROMYCES-CEREVISIAE; PRERIBOSOMAL RNA; SECONDARY STRUCTURE; PREMESSENGER RNA; INTRON; METHYLATION; GENES; SITE;
Keywords:
ribonuclease; endonuclease; snoRNA; nucleolus; tetraloop;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
48
Recensione:
Indirizzi per estratti:
Indirizzo: Chanfreau, G Inst,Pasteur, Lab Metab ARN, CNRS, URA 1300,Dept Biotechnol, 25 Rue Dr Roux Inst Pasteur 25 Rue Dr Roux Paris France 15 , 25 Rue Dr Roux
Citazione:
G. Chanfreau et al., "Yeast RNase III as a key processing enzyme in small nucleolar RNAs metabolism", J MOL BIOL, 284(4), 1998, pp. 975-988

Abstract

The variety of biogenesis pathways for small nucleolar RNAs (snoRNAs) reflects the diversity of their genomic organization. We have searched for yeast snoRNAs which are affected by the depletion of the yeast ortholog of bacterial RNase III, Rnt1. In a yeast strain inactivated for RNT1, almost half of the snoRNAs tested are depleted with significant accumulation of monocistronic or polycistronic precursors. snoRNAs from both major families of snoRNAs (C/D and H/ACA) are affected by RNT1 disruption. In vitro, recombinantRnt1 specifically cleaves pre-snoRNA precursors in the absence of other factors, generating intermediates which require the action of other enzymes for processing to the mature snoRNA. Most Rnt1 cleavage sites fall within potentially double-stranded regions closed by tetraloops with a novel consensus sequence AGNN. These results demonstrate that biogenesis of a large number of snoRNAs from the two major families of snoRNAs requires a common RNA endonuclease and a putative conserved structural motif. (C) 1998 Academic Press.

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Documento generato il 04/12/20 alle ore 13:25:02