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Titolo:
Leaf inner structure and immunogold localization of some key enzymes involved in carbon metabolism in CAM plants
Autore:
Kondo, A; Nose, A; Ueno, O;
Indirizzi:
Natlapant Agrobiol Resources, Dept Plant Physiol, Ibaraki, Osaka 3058602, J Natl Inst Agrobiol Resources Ibaraki Osaka Japan 3058602 Osaka 3058602, J Saga Univ, Fac Agr, Saga 8408502, Japan Saga Univ Saga Japan 8408502Saga Univ, Fac Agr, Saga 8408502, Japan
Titolo Testata:
JOURNAL OF EXPERIMENTAL BOTANY
fascicolo: 329, volume: 49, anno: 1998,
pagine: 1953 - 1961
SICI:
0022-0957(199812)49:329<1953:LISAIL>2.0.ZU;2-7
Fonte:
ISI
Lingua:
ENG
Soggetto:
CRASSULACEAN-ACID-METABOLISM; MESEMBRYANTHEMUM-CRYSTALLINUM; IMMUNOCYTOCHEMICAL LOCALIZATION; PHOTOSYNTHETIC CHARACTERISTICS; KALANCHOE-DAIGREMONTIANA; ORTHOPHOSPHATE DIKINASE; PEPEROMIA-CAMPTOTRICHA; ELEOCHARIS-VIVIPARA; TISSUE LAYERS; PYRUVATE;
Keywords:
Crassulacean acid metabolism; immunolocalization; leaf inner structure; phosphoenolpyruvate carboxylase; pyruvate orthophosphate dikinase;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Agriculture,Biology & Environmental Sciences
Life Sciences
Citazioni:
33
Recensione:
Indirizzi per estratti:
Indirizzo: Ueno, O Natlapant Agrobiol Resources, Dept Plant Physiol, Ibaraki, Osaka 3058602, J Natl Inst Agrobiol Resources Ibaraki Osaka Japan 3058602 58602, J
Citazione:
A. Kondo et al., "Leaf inner structure and immunogold localization of some key enzymes involved in carbon metabolism in CAM plants", J EXP BOT, 49(329), 1998, pp. 1953-1961

Abstract

There are relatively few reports on the leaf structure and in situ immunolocalization of carbon metabolism enzymes in crassulacean acid metabolism (CAM) plants, compared with reports on C-4 plants. The leaf inner structure and the subcellular location of some key CAM enzymes for a phosphoenolpyruvate carboxykinase (PCK) CAM species, Ananas comosus, and three malic enzyme (ME) CAM species, Mesembryanthemum crystallinum, Kalanchoe daigremontiana, and K. pinnata, was investigated by immunogold labelling and electron microscopy in this study. The leaves of these species had few intercellular air spaces in the mesophyll. A large vacuole occupied the mesophyll cells, and many vesicles of various sizes occurred in the cytosol. Immunocytochemical study revealed that labelling was present for phosphoenolpyruvate carboxylase in the cytosol and for ribulose-1,5-bisphosphate carboxylase/oxygenase in the chloroplasts of the mesophyll cells in all species. No specific labelling for pyruvate orthophosphate dikinase (PPDK) was observed in the PCK-CAM species. In the ME-CAM species, the patterns of labelling for PPDK differed. In M. crystallinum labelling for PPDK was present only in the chloroplasts, whereas in the two Kalanchoe species it occurred in the cytosol as well as in the chloroplasts. These results suggest that the subcellular localization of PPDK varies with ME-CAM species, in contrast to the conventional belief that it is localized in the chloroplasts.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 10/07/20 alle ore 03:16:53