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Titolo:
Processing of the psbA 5 ' untranslated region in Chlamydomonas reinhardtii depends upon factors mediating ribosome association
Autore:
Bruick, RK; Mayfield, SP;
Indirizzi:
Scripps Clin & Res Inst, Dept Cell Biol, La Jolla, CA 92037 USA Scripps Clin & Res Inst La Jolla CA USA 92037 iol, La Jolla, CA 92037 USA Scripps Clin & Res Inst, Skaggs Inst Chem Biol, La Jolla, CA 92037 USA Scripps Clin & Res Inst La Jolla CA USA 92037 iol, La Jolla, CA 92037 USA
Titolo Testata:
JOURNAL OF CELL BIOLOGY
fascicolo: 5, volume: 143, anno: 1998,
pagine: 1145 - 1153
SICI:
0021-9525(19981130)143:5<1145:POTP5'>2.0.ZU;2-K
Fonte:
ISI
Lingua:
ENG
Soggetto:
CHLOROPLAST GENE-EXPRESSION; LIGHT-REGULATED TRANSLATION; THYLAKOID MEMBRANE-PROTEIN; MESSENGER-RNA; PHOTOSYSTEM-II; NUCLEOTIDE-SEQUENCE; NUCLEAR; TRANSCRIPTION; BARLEY; STABILITY;
Keywords:
translation; mRNA processing; chloroplast; ribosome binding sequence; psbA;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
51
Recensione:
Indirizzi per estratti:
Indirizzo: Mayfield, SP Scripps Clin & Res Inst, Dept Cell Biol, 10550 N Torrey PinesRd, La Jolla, Scripps Clin & Res Inst 10550 N Torrey Pines Rd La Jolla CA USA 92037
Citazione:
R.K. Bruick e S.P. Mayfield, "Processing of the psbA 5 ' untranslated region in Chlamydomonas reinhardtii depends upon factors mediating ribosome association", J CELL BIOL, 143(5), 1998, pp. 1145-1153

Abstract

The 5' untranslated region of the chloroplast psbA mRNA, encoding the D1 protein, is processed in Chlamydomonas reinhardtii. Processing occurs just upstream of a consensus Shine-Dalgarno sequence and results in the removal of 54 nucleotides from the 5' terminus, including a stem-loop element identified previously as an important structure for D1 expression. Examination ofthis processing event in C. reinhardtii strains containing mutations within the chloroplast or nuclear genomes that block psbA translation reveals a correlation between processing and ribosome association. Mutations within the 5' untranslated region of the psbA mRNA that disrupt the Shine-Dalgarno sequence, acting as a ribosome binding site, preclude translation and prevent mRNA processing. Similarly, nuclear mutations that specifically affect synthesis of the D1 protein specifically affect processing of the psbA mRNA. In vitro, loss of the stem-loop element does not prohibit the binding of amessage-specific protein complex required for translational activation of psbA upon illumination. These results are consistent with a hierarchical maturation pathway for chloroplast messages, mediated by nuclear-encoded factors, that integrates mRNA processing, message stability, ribosome association, and translation.

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Documento generato il 30/11/20 alle ore 06:25:19