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Titolo:
Characterization of latex allergenic components by capillary zone electrophoresis and N-terminal sequence analysis
Autore:
Chen, HD; Chen, CL; Huang, SW; Kung, HF; Chen, HC;
Indirizzi:
NCI,erick,rick Canc Res & Dev Ctr, Lab Biochem Physiol, Div Basic Sci, Fred NCI Frederick MD USA 21701 Ctr, Lab Biochem Physiol, Div Basic Sci, Fred Natl Chung Hsing Univ, Dept Vet Med, Taichung 40227, Taiwan Natl Chung Hsing Univ Taichung Taiwan 40227 Med, Taichung 40227, Taiwan Univ Florida, Coll Vet Med, Dept Large Anim Clin Sci, Gainesville, FL 32610 Univ Florida Gainesville FL USA 32610 nim Clin Sci, Gainesville, FL 32610 Univ Florida, Coll Med, Dept Pediat, Gainesville, FL USA Univ Florida Gainesville FL USA ll Med, Dept Pediat, Gainesville, FL USA NICHHD, Endocrinol & Reprod Res Branch, NIH, Bethesda, MD 20892 USA NICHHD Bethesda MD USA 20892 prod Res Branch, NIH, Bethesda, MD 20892 USA
Titolo Testata:
JOURNAL OF BIOMEDICAL SCIENCE
fascicolo: 6, volume: 5, anno: 1998,
pagine: 421 - 427
SICI:
1021-7770(199811/12)5:6<421:COLACB>2.0.ZU;2-0
Fonte:
ISI
Lingua:
ENG
Soggetto:
RUBBER ELONGATION-FACTOR; HEVEA-BRASILIENSIS; CONTACT URTICARIA; MAJOR ALLERGEN; IDENTIFICATION; PROTEINS; PROHEVEIN; GLOVES;
Keywords:
latex; capillary zone electrophoresis; sequence; hevein;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
24
Recensione:
Indirizzi per estratti:
Indirizzo: Chen, HD NCI, Frederick Canc Res & Dev Ctr, Lab Biochem Physiol, Div BasicSci, Bldg NCI Bldg 567,Rm 152 Frederick MD USA 21701 l, Div Basic Sci, Bldg
Citazione:
H.D. Chen et al., "Characterization of latex allergenic components by capillary zone electrophoresis and N-terminal sequence analysis", J BIOMED SC, 5(6), 1998, pp. 421-427

Abstract

In a previous study, protein components purified from latex gloves that elicited allergenic reactions were characterized by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE), and yielded apparent molecularweights of 14, 22, 30, 34, 46, and 58 kD, These allergenic components wereisolated for further characterization by capillary zone electrophoresis and N-terminal amino acid sequence analysis. These components all migrated atapproximately 25 and 35 min on capillary zone electrophoresis, Diode arrayspectral analysis detected indistinguishable characteristics between thesetwo protein peaks, In addition, complex formation of these components withpatients' immunoglobulin was demonstrated by capillary zone electrophoresis. Analysis of components separated by SDS-PAGE on a polyvinylidene difluoride membrane showed that the first 13 residues were identical to the sequence of hevein, Based on the criteria of charge-to-mass ratio and N-terminal amino acid sequence, our results suggest that these components of latex proteins are similar in the primary structure.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 19/01/20 alle ore 20:33:10