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Titolo:
Responses of broccoli seedlings to light quality during low-temperature storage in vitro: I. Morphology and survival
Autore:
Wilson, SB; Iwabuchi, K; Rajapakse, NC; Young, RE;
Indirizzi:
Clemson Univ, Dept Hort, Clemson, SC 29634 USA Clemson Univ Clemson SC USA 29634 Univ, Dept Hort, Clemson, SC 29634 USA Clemson Univ, Dept Agr & Biol Engn, Clemson, SC 29634 USA Clemson Univ Clemson SC USA 29634 Agr & Biol Engn, Clemson, SC 29634 USA
Titolo Testata:
HORTSCIENCE
fascicolo: 7, volume: 33, anno: 1998,
pagine: 1253 - 1257
SICI:
0018-5345(199812)33:7<1253:ROBSTL>2.0.ZU;2-W
Fonte:
ISI
Lingua:
ENG
Soggetto:
GROWTH SUPPRESSION; PLANTLETS; PRESERVATION; TRANSPLANTS; EFFICIENCY; CUTTINGS; RED;
Keywords:
Brassica oleracea; postharvest physiology; spectral quality;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Agriculture,Biology & Environmental Sciences
Citazioni:
27
Recensione:
Indirizzi per estratti:
Indirizzo: Rajapakse, NC Clemson Univ, Dept Hort, Clemson, SC 29634 USA Clemson UnivClemson SC USA 29634 rt, Clemson, SC 29634 USA
Citazione:
S.B. Wilson et al., "Responses of broccoli seedlings to light quality during low-temperature storage in vitro: I. Morphology and survival", HORTSCIENCE, 33(7), 1998, pp. 1253-1257

Abstract

Broccoli (Brassica oleracea L. Botrytis group 'Green Duke') seeds were cultured in vitro photoautotrophically (without sugar in the medium) or photomixotrophically (with sugar in the medium) for 3 weeks at 23 degrees C and 158 mu mol.m(-2).s(-1) photosynthetic photon flux (PPF), Vessels were then stored at 5 degrees C under 1.6, 4.1, or 8.6 mu mol.m(-2).s(-1) of white (400-800 nm), red (600-700 nm), or blue (400-500 nm) light. Concentrations of CO, inside the vessels were monitored until equilibrium was reached. Light compensation point was reached at 3.5 mu mol.m(-2).s(-1) for photoautotrophic seedlings and at 6.5 mu mol.m(-2).s(-1) for photomixotrophic seedlings. Therefore, in the long-term storage experiment, seedlings were stored for 4, 8, or 12 weeks at 5 degrees C in darkness or under 5 mu mol.m(-2).s(-1) (average light compensation point) of white, red, or blue light. Illumination during storage was necessary to maintain dry mass, leaf area, and regrowth potentials of in vitro seedlings. All seedlings stored in darkness were of poor quality and died when transferred to the greenhouse. Red light during storage increased seedling dry mass and chlorophyll content and improved overall appearance, whereas blue light decreased chlorophyll content and increased stem elongation. The addition of 2% sucrose to media increased dry mass and leaf area and maintained overall seedling quality during illuminated storage, However, plantlets stored for more than 4 weeks did not survivepoststorage greenhouse conditions, regardless of light treatment.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 03/04/20 alle ore 03:28:44