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Titolo:
Characterization of oligosaccharides from an antigenic mannan of Saccharomyces cerevisiae
Autore:
Young, M; Davies, MJ; Bailey, D; Gradwell, MJ; Smestad-Paulsen, B; Wold, JK; Barnes, RMR; Hounsell, EF;
Indirizzi:
Univ Coll London, Dept Biochem & Mol Biol, London WC1E 6BT, England Univ Coll London London England WC1E 6BT Biol, London WC1E 6BT, England Natl Inst Med Res, MRC, Biomed NMR Ctr, London NW7 1AA, England Natl Inst Med Res London England NW7 1AA MR Ctr, London NW7 1AA, England Univ Oslo, Inst Pharm, N-0316 Oslo, Norway Univ Oslo Oslo Norway N-0316Univ Oslo, Inst Pharm, N-0316 Oslo, Norway Univ Liverpool, Dept Immunol, Liverpool L69 3BX, Merseyside, England Univ Liverpool Liverpool Merseyside England L69 3BX , Merseyside, England
Titolo Testata:
GLYCOCONJUGATE JOURNAL
fascicolo: 8, volume: 15, anno: 1998,
pagine: 815 - 822
SICI:
0282-0080(199808)15:8<815:COOFAA>2.0.ZU;2-7
Fonte:
ISI
Lingua:
ENG
Soggetto:
INFLAMMATORY BOWEL-DISEASE; BAKERS-YEAST; CROHNS-DISEASE; GLYCOPROTEIN ANTIGEN; SERUM ANTIBODIES; CANDIDA-ALBICANS; IGE-BINDING; IN-VITRO; SPECTROSCOPY; ALLERGENS;
Keywords:
Saccharomyces cerevisiae; oligosaccharide structure; antigenic glycoprotein; mannan; allergens;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
32
Recensione:
Indirizzi per estratti:
Indirizzo: Hounsell, EF Univndoll London, Dept Biochem & Mol Biol, Gower St, London WC1E 6BT, Engla Univ Coll London Gower St London England WC1E 6BT 6BT, Engla
Citazione:
M. Young et al., "Characterization of oligosaccharides from an antigenic mannan of Saccharomyces cerevisiae", GLYCOCON J, 15(8), 1998, pp. 815-822

Abstract

Mannans of the yeast Saccharomyces cerevisiae have been implicated as containing the allergens to which bakers and brewers are sensitive and also theantigen recognized by patients with Crohn's disease. A fraction of S. cerevisiae mannan, Sc500, having high affinity for antibodies in Crohn's patients has been characterized by NMR spectroscopy followed by fragmentation using alkaline elimination, partial acid hydrolysis and acetolysis. The released oligosaccharides were separated by gel filtration on a Biogel P4 column and analyzed by fluorescence labeling, HPLC and methylation analysis. The relationship between structure and antigen activity was measured by competitive ELISA, The antigenic activity of the original high molecular weight mannan could be ascribed to terminal Man alpha 1 --> 3Man alpha 1 --> 2 sequences which are rarely found in human glycoproteins but were over-representedin Sc500 compared to other yeast mannans.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 28/09/20 alle ore 12:12:21