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Titolo:
Identification of Cannabis pollens using an allergic patient's immunoglobulin E and purification and characterization of allergens in Cannabis pollens
Autore:
Tanaka, H; Degawa, M; Kawata, E; Hayashi, J; Shoyama, Y;
Indirizzi:
Kyushu2,niv, Fac Pharmaceut Sci, Dept Pharmacognosy, Higashi Ku, Fukuoka 81 Kyushu Univ Fukuoka Japan 812 Dept Pharmacognosy, Higashi Ku, Fukuoka 81 Kyushu Univ, Fac Med, Dept Gen Med, Higashi Ku, Fukuoka 812, Japan Kyushu Univ Fukuoka Japan 812 pt Gen Med, Higashi Ku, Fukuoka 812, Japan
Titolo Testata:
FORENSIC SCIENCE INTERNATIONAL
fascicolo: 2-3, volume: 97, anno: 1998,
pagine: 139 - 153
SICI:
0379-0738(19981109)97:2-3<139:IOCPUA>2.0.ZU;2-L
Fonte:
ISI
Lingua:
ENG
Soggetto:
IONIZATION MASS-SPECTROMETRY; ARTEMISIA-VULGARIS L; MAJOR ALLERGEN; GLYCOPROTEIN ALLERGEN; MONOCLONAL-ANTIBODIES; ENZYME-IMMUNOASSAY; PLANT-TISSUE; ABBOTT-ADX; GC-MS; ACID;
Keywords:
Cannabis sativa; marihuana; pollen; identification; allergen; Western blotting; ELISA;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Clinical Medicine
Citazioni:
45
Recensione:
Indirizzi per estratti:
Indirizzo: Shoyama, Y Kyushuashi,, Fac Pharmaceut Sci, Dept Pharmacognosy, Higashi Ku, 3-1-1 Maid Kyushu Univ 3-1-1 Maidashi Fukuoka Japan 812 hi Ku, 3-1-1 Maid
Citazione:
H. Tanaka et al., "Identification of Cannabis pollens using an allergic patient's immunoglobulin E and purification and characterization of allergens in Cannabis pollens", FOREN SCI I, 97(2-3), 1998, pp. 139-153

Abstract

Cannabis pollen allergens were detected using the serum of an allergic patient. The allergens were then purified by sequential column chromatography (including DE52 cellulose and phenyl-Sepharose CL-4B) and preparative HPLC. The molecular weight of the allergens were determined as 10 050 and 13 706by matrix-assisted laser desorption/ionization time of flight mass spectrometry. We utilised Western blotting and development of an enzyme-linked immunosorbent assay for the detection of Cannabis pollen allergens. (C) 1998 Elsevier Science Ireland Ltd All rights reserved.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 31/10/20 alle ore 00:38:48