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Titolo:
Human preformed IgG combining with membrane-bound porcine serotransferrin lyse porcine endothelial cells through antibody-dependent cellular cytotoxicity
Autore:
Atia, N; Camoin, L; Duflos, G; Malassagne, B; Chereau, C; Filipponi, F; Mahboub, S; Batteux, F; Conti, F; Michalski, JC; Houssin, D; Calmus, Y; Weill, B;
Indirizzi:
Univ Paris 05, Fac Cochin, Immunol Lab, Paris, France Univ Paris 05 Paris France s 05, Fac Cochin, Immunol Lab, Paris, France Insts,ochin Genet Mol, CNRS, UPR 415, Lab Immunopharmacol Mol, F-75014 Pari Inst Cochin Genet Mol Paris France F-75014 nopharmacol Mol, F-75014 Pari Univeuve & Tech Lille Flandres Artois, Chim Biol Lab, CNRS, UMR 111, Villen Univ Sci & Tech Lille Flandres Artois Villeneuve Dascq France 1, Villen Univ Paris 05, Fac Cochin, Lab Rech Chirurg, Paris, France Univ Paris 05 Paris France Fac Cochin, Lab Rech Chirurg, Paris, France Osped Casanello, Ist Chirurg Gen & Esperimentale, Pisa, Italy Osped Casanello Pisa Italy Ist Chirurg Gen & Esperimentale, Pisa, Italy Univ Paris 05, Biol Cellulaire Lab, Fac Cochin, Paris, France Univ Paris 05 Paris France ol Cellulaire Lab, Fac Cochin, Paris, France
Titolo Testata:
EUROPEAN JOURNAL OF IMMUNOLOGY
fascicolo: 12, volume: 28, anno: 1998,
pagine: 3917 - 3928
SICI:
0014-2980(199812)28:12<3917:HPICWM>2.0.ZU;2-K
Fonte:
ISI
Lingua:
ENG
Soggetto:
GAS-LIQUID-CHROMATOGRAPHY; NATURAL ANTIBODIES; MEDIATED CYTOTOXICITY; TRANSFERRIN RECEPTOR; XENOGRAFT REJECTION; MASS-SPECTROMETRY; METHYL GLYCOSIDES; OLIGOSACCHARIDES; GLYCOPROTEINS; RECOGNITION;
Keywords:
preformed IgG; porcine serotransferrin; endothelial cell; antibody-dependent cellular cytotoxicity;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
35
Recensione:
Indirizzi per estratti:
Indirizzo: Weill, B Hop Cochin, Immunol Lab, Pavillon Hardy,27 Rue Fg St Jacques, F-75674 Paris Hop Cochin Pavillon Hardy,27 Rue Fg St Jacques Paris France 14 s
Citazione:
N. Atia et al., "Human preformed IgG combining with membrane-bound porcine serotransferrin lyse porcine endothelial cells through antibody-dependent cellular cytotoxicity", EUR J IMMUN, 28(12), 1998, pp. 3917-3928

Abstract

Preformed antibodies are involved in xenograft rejection. The purpose of this work was to characterize porcine xenoantigens recognized by human preformed IgG (hplgG), and to investigate the role of hplgG in xenogeneic rejection. IgG eluted from porcine livers perfused with human plasma, human sera and total human IgG were immunoblotted on porcine aortic endothelial cell extracts. The amino acid sequence of a 76-kDa antigen constantly revealed was 100 % homologous with porcine serotransferrin (psTf). hplgG from human sera, human IgG1 and IgG2 and F(ab')(2)gamma specifically bound to psTf. Neutralization by psTf abolished that binding. Although alpha 1,3-linked galactose residues (Gal alpha 1,3Gal) is the dominant epitope recognized by preformed antibodies in the swine-to-human combination, the analysis of carbohydrate composition of psTf showed that the molecule was devoid of Gal alpha 1,3Gal moieties and that preformed anti-psTf IgG bound to epitopes localizedon the peptide core of the molecule. Purified human anti-psTf IgG antibodies were able to bind to psTf linked to its receptor on porcine endothelial cells, and to kill those cells through antibody-dependent cellular cytotoxicity.

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Documento generato il 03/07/20 alle ore 22:15:36