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Titolo:
Detection of SYT-SSX1/2 fusion transcripts by reverse transcriptase-polymerase chain reaction (RT-PCR) is a valuable diagnostic tool in synovial sarcoma
Autore:
Willeke, F; Mechtersheimer, G; Schwarzbach, M; Weitz, J; Zimmer, D; Lehnert, T; Herfarth, C; Doeberitz, MV; Ridder, R;
Indirizzi:
Univ Heidelberg, Dept Surg, Div Mol Diagnost & Therapy, D-69120 Heidelberg, Univ Heidelberg Heidelberg Germany D-69120 Therapy, D-69120 Heidelberg, Univ Heidelberg, Inst Pathol, D-69120 Heidelberg, Germany Univ HeidelbergHeidelberg Germany D-69120 , D-69120 Heidelberg, Germany Univ Heidelberg, Dept Surg, Div Surg Oncol, D-69120 Heidelberg, Germany Univ Heidelberg Heidelberg Germany D-69120 , D-69120 Heidelberg, Germany
Titolo Testata:
EUROPEAN JOURNAL OF CANCER
fascicolo: 13, volume: 34, anno: 1998,
pagine: 2087 - 2093
SICI:
0959-8049(199812)34:13<2087:DOSFTB>2.0.ZU;2-I
Fonte:
ISI
Lingua:
ENG
Soggetto:
IN-SITU HYBRIDIZATION; SOFT-TISSUE SARCOMAS; CHROMOSOMAL TRANSLOCATIONS; SYT; GENES; T(X-18); ASSAYS; IDENTIFICATION; PATHOLOGY; PROTEINS;
Keywords:
sarcoma; translocation; RT-PCR; minimal residual disease; SYT-SSX1/2;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Clinical Medicine
Life Sciences
Citazioni:
35
Recensione:
Indirizzi per estratti:
Indirizzo: Willeke, F Univ0,eidelberg, Dept Surg, Div Mol Diagnost & Therapy, Neuenheimer Feld 11 Univ Heidelberg Neuenheimer Feld 110 Heidelberg Germany D-69120
Citazione:
F. Willeke et al., "Detection of SYT-SSX1/2 fusion transcripts by reverse transcriptase-polymerase chain reaction (RT-PCR) is a valuable diagnostic tool in synovial sarcoma", EUR J CANC, 34(13), 1998, pp. 2087-2093

Abstract

Cytogenetically, most synovial sarcomas are characterised by a specific chromosomal translocation [(X;18)(p11.2;q11.2)], which results in the generation of fusion transcripts comprising SYT (18q11) and either SSX1 or SSX2 (Xp11) sequences. By using a sensitive reverse transcriptase-polymerase chainreaction (RT-PCR) protocol, specific SYT-SSX1/2 fusion transcripts were detected in 10 histopathologically confirmed synovial sarcomas. Control tumours with morphological spindle cell patterns mimicking monophasic synovial sarcoma tested negative (18/19) in the RT-PCR protocol, with the exception of one spindle cell sarcoma originally classified as a fibrosarcoma. Furthermore, the established RT-PCR protocol was used to evaluate the feasibility of SYT-SSX1/2 fusion transcript detection for minimal residual disease analysis. Analyses of surgical margins revealed a fusion transcript in two of four operations for synovial sarcoma analysed, one of which was diagnosed with tumour free margins by conventional histopathology. These data suggest that the RT-PCR amplification of SYT-SSX1/2 fusion transcripts is a valuabletool in the differentiation of synovial sarcomas, especially in cases of equivocal morphology. Additionally, the RT-PCR approach may be used for the detection of residual tumour cells in synovial sarcoma patients. (C) 1998 Elsevier Science Ltd. All rights reserved.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 28/09/20 alle ore 15:39:10