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Titolo:
Immunoelectron microscopic localization of nitric oxide synthase III in the guinea pig organ of Corti
Autore:
Heinrich, UR; Maurer, J; Gosepath, K; Mann, W;
Indirizzi:
Univ Mainz, ENT Dept, D-55131 Mainz, Germany Univ Mainz Mainz Germany D-55131 Mainz, ENT Dept, D-55131 Mainz, Germany
Titolo Testata:
EUROPEAN ARCHIVES OF OTO-RHINO-LARYNGOLOGY
fascicolo: 10, volume: 255, anno: 1998,
pagine: 483 - 490
SICI:
0937-4477(199811)255:10<483:IMLONO>2.0.ZU;2-F
Fonte:
ISI
Lingua:
ENG
Soggetto:
OUTER HAIR-CELLS; COCHLEA; ACTIN; MEMBRANE; CHANNELS; MOTILITY; CALCIUM;
Keywords:
sound transduction; cochlear hair cells; immunocytochemistry; nitric oxide synthase; guinea pig;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Clinical Medicine
Citazioni:
23
Recensione:
Indirizzi per estratti:
Indirizzo: Heinrich, UR Univ Mainz, ENT Dept, Langenbeckstr 1, D-55131 Mainz, GermanyUniv Mainz Langenbeckstr 1 Mainz Germany D-55131 nz, Germany
Citazione:
U.R. Heinrich et al., "Immunoelectron microscopic localization of nitric oxide synthase III in the guinea pig organ of Corti", EUR ARCH OT, 255(10), 1998, pp. 483-490

Abstract

Nitric oxide synthase III (NOS III) was identified in the guinea pig cochlea on an ultrastructural level using a post-embedding immunolabeling procedure. Ultrathin sections of London Resin (LR) White-embedded specimens were incubated with various concentrations of a commercially available antibody to NOS III and the immunoreactivity visualized by a gold-labeled secondary antibody. Analysis of ultrathin sections of the organ of Corti in the second turn of the cochlea showed that NOS III could be localized in the endothelial cells of the blood vessels under the basilar membrane, which was comparable to its location in similar cells types in various biological systems. Besides this, NOS III was also found in the cytoplasm and in the nuclei ofinner and outer hair cells. Immunoreactivity was not distributed homogeneously within receptor cells. Numerous gold particles could be identified at the border of the cuticular plates, in the middle parts of the stereocilia and in the cytoplasm. Gold-labeled anti-NOS III antibodies in these sites were seen mostly on the cytoplasmic side of the submembranous cisterns in the vicinity of mitochondria and in the central parts of the hair cells, whereas the cisterns were nearly free from any immunoreactivity. NOS III was also detected in the efferent and afferent nerve endings that were located atthe basal and basolateral side of the outer hair cells. Some immunoreactivity was visible in different nerve fibers of the inner and outer spiral tunnels. Besides this, gold-labeled antibodies were also present in the cuticular plate of inner and outer pillar cells, in the cytoskeletal elements located in the apical parts of Deiters cells, forming the lamina reticularis, and in the cytoskeletal-containing region of the cytoplasm of those Deiterscells located at the basal side of the outer hair cells. The role of the NOS III immunoreactivity identified in the organ of Corti was consistent with respect to hair cell and tissue modulation.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 25/11/20 alle ore 07:06:42