Catalogo Articoli (Spogli Riviste)

OPAC HELP

Titolo:
Integrins induce activation of EGF receptor: role in MAP kinase induction and adhesion-dependent cell survival
Autore:
Moro, L; Venturino, M; Bozzo, C; Silengo, L; Altruda, F; Beguinot, L; Tarone, G; Defilippi, P;
Indirizzi:
Univ Turin, Dipartimento Genet Biol & Biochim, I-10126 Turin, Italy Univ Turin Turin Italy I-10126 enet Biol & Biochim, I-10126 Turin, Italy Hosp San Raffaele, Inst Neurosci & Bioimmagini, I-20132 Milan, Italy Hosp San Raffaele Milan Italy I-20132 Bioimmagini, I-20132 Milan, Italy DIBIT, Unita Oncol Mol, Milan, Italy DIBIT Milan ItalyDIBIT, Unita Oncol Mol, Milan, Italy Dipartimento Sci Med, Novara, Italy Dipartimento Sci Med Novara ItalyDipartimento Sci Med, Novara, Italy
Titolo Testata:
EMBO JOURNAL
fascicolo: 22, volume: 17, anno: 1998,
pagine: 6622 - 6632
SICI:
0261-4189(19981116)17:22<6622:IIAOER>2.0.ZU;2-Q
Fonte:
ISI
Lingua:
ENG
Soggetto:
GROWTH-FACTOR RECEPTOR; GLOMERULAR EPITHELIAL-CELLS; HUMAN ENDOTHELIAL-CELLS; PDGF-BETA-RECEPTORS; TYROSINE PHOSPHORYLATION; EXTRACELLULAR-MATRIX; SIGNAL-TRANSDUCTION; PROTEIN-KINASE; CYCLE PROGRESSION; PP125(FAK);
Keywords:
adhesion-dependent cell survival; cell-matrix interaction; EGF receptor; integrin signaling; MAP kinase;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
64
Recensione:
Indirizzi per estratti:
Indirizzo: Defilippi, P Univ Turin, Dipartimento Genet Biol & Biochim, I-10126 Turin,Italy Univ Turin Turin Italy I-10126 iochim, I-10126 Turin, Italy
Citazione:
L. Moro et al., "Integrins induce activation of EGF receptor: role in MAP kinase induction and adhesion-dependent cell survival", EMBO J, 17(22), 1998, pp. 6622-6632

Abstract

Adhesion of human primary skin fibroblasts and ECV304 endothelial cells toimmobilized matrix proteins, beta 1 or alpha v integrin antibodies stimulates tyrosine phosphorylation of the epidermal growth factor (EGF) receptor. This tyrosine phosphorylation is transiently induced, reaching maximal levels 30 min after adhesion, and it occurs in the absence of receptor ligands. Similar results were observed with EGF receptor-transfected NIH-3T3 cells. Use of a kinase-negative EGF receptor mutant demonstrates that the integrin-stimulated tyrosine phosphorylation is due to activation of the receptor's intrinsic kinase activity. Integrin-mediated EGF receptor activation leads to Erk-1/MAP kinase induction, as shown by treatment with the specific inhibitor tyrphostin AG1478 and by expression of a dominant-negative EGF receptor mutant. EGF receptor and Erk-1/MAP kinase activation by integrins does not lead per se to cell proliferation, but is important for entry into S phase in response to EGF or serum. EGF receptor activation is also requiredfor extracellular matrix-mediated cell survival. Adhesion-dependent R IAP kinase activation and survival are regulated through EGF receptor activation in cells expressing this molecule above a threshold level (5 x 10(3) receptors per cell). These results demonstrate that integrin-dependent EGF receptor activation is a novel signaling mechanism involved in cell survival and proliferation in response to extracellular matrix.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 07/07/20 alle ore 06:20:09