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Titolo:
Inhibition of glucose stimulated insulin secretion by neuropeptide Y is mediated via the Y1 receptor and inhibition of adenylyl cyclase in RIN 5AH rat insulinoma cells
Autore:
Morgan, DG; Kulkarni, RN; Hurley, JD; Wang, ZL; Wang, RM; Ghatei, MA; Karlsen, AE; Bloom, SR; Smith, DM;
Indirizzi:
UnivMLondon Imperial Coll Sci Technol & Med, Sch Med, Hammersmith Hosp, ICS Univ London Imperial Coll Sci Technol & Med London England W12 0HH , ICS Harvardon,iv, Sch Med, Joslin Diabet Ctr, Dept Cellular & Mol Physiol, Bost Harvard Univ Boston MA USA 02115 t Ctr, Dept Cellular & Mol Physiol, Bost Harvard Univ, Inst Med, Dept Expt Med, Boston, MA 02115 USA Harvard Univ Boston MA USA 02115 Med, Dept Expt Med, Boston, MA 02115 USA Steno Diabet Ctr, DK-2820 Gentofte, Denmark Steno Diabet Ctr Gentofte Denmark DK-2820 Ctr, DK-2820 Gentofte, Denmark
Titolo Testata:
DIABETOLOGIA
fascicolo: 12, volume: 41, anno: 1998,
pagine: 1482 - 1491
SICI:
0012-186X(199812)41:12<1482:IOGSIS>2.0.ZU;2-P
Fonte:
ISI
Lingua:
ENG
Soggetto:
ISLET AMYLOID POLYPEPTIDE; PEPTIDE-YY; FUNCTIONAL EXPRESSION; DEXAMETHASONE TREATMENT; PANCREATIC-POLYPEPTIDE; GLUCAGON-SECRETION; PARACRINE ROLE; G-PROTEIN; CLONING; RELEASE;
Keywords:
neuropeptide Y; Y1 receptor; insulin secretion; insulinoma cells;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Clinical Medicine
Life Sciences
Citazioni:
44
Recensione:
Indirizzi per estratti:
Indirizzo: Smith, DM UnivMLondon Imperial Coll Sci Technol & Med, Sch Med, Hammersmith Hosp, ICS Univ London Imperial Coll Sci Technol & Med Du Cane Rd London England W12 0HH
Citazione:
D.G. Morgan et al., "Inhibition of glucose stimulated insulin secretion by neuropeptide Y is mediated via the Y1 receptor and inhibition of adenylyl cyclase in RIN 5AH rat insulinoma cells", DIABETOLOG, 41(12), 1998, pp. 1482-1491

Abstract

Neuropeptide Y (NPY) has been shown to inhibit insulin secretion from the islets of Langerhans. We show that insulin secretion in the insulinoma cellline RIN 5AH is inhibited by NPY. I-125-Peptide YY (PYY) saturation and competition-binding studies using NPY fragments and analogues on membranes prepared from this cell line show the presence of a single class of NPY receptor with a Y1 receptor subtype-like profile. Inhibition of insulin secretion in this cell line by NPY fragments and analogues also shows a Y1 receptor-like profile. Both receptor binding and inhibition of insulin secretion showed the same orders of potency with NPY > [Pro(34)] NPY > NPY 3-36 > > NPY13-36. The Y1 receptor antagonist, BIBP 3226, blocks NPY inhibition of insulin secretion from, and inhibits I-125-PYY binding to, RIN 5AH cells. Northern blot analysis using a Y1-receptor specific probe shows that NPY Y1 receptors are expressed by RIN 5AH cells. Y5 receptors are not expressed in this cell line. Neuropeptide Y inhibition of insulin secretion is blocked by incubation with pertussis toxin, implying that the effect is via a G-protein (G(i) or G(o)) coupled receptor. Neuropeptide Y inhibits the activation of adenylyl cyclase by isoprenaline in RIN 5AH. cell lysates, and the stimulation of cAMP by glucagon-like peptide-1 (7-36) amide (GLP-1). It also blocks insulin secretion stimulated by GLP-1, but not by dibutyryl cyclic AMP. Hence, we suggest that NPY inhibits insulin secretion from RIN SAH cells via a Y1 receptor linked through G(i) to the inhibition of adenylyl cyclase.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 01/04/20 alle ore 11:46:33