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Titolo:
Evidence of juxtacrine signaling for transforming growth factor alpha in human endometrium
Autore:
Bush, MR; Mele, JM; Couchman, GM; Walmer, DK;
Indirizzi:
Duke,Univ, Med Ctr, Dept Obstet & Gynecol, Div Reprod Endocrinol & Infertil Duke Univ Durham NC USA 27710 & Gynecol, Div Reprod Endocrinol & Infertil NIEHS, Mol Carcinogenesis Lab, NIH, Res Triangle Pk, NC 27709 USA NIEHS Res Triangle Pk NC USA 27709 ab, NIH, Res Triangle Pk, NC 27709 USA NIEHS, Reprod & Dev Toxicol Lab, NIH, Res Triangle Pk, NC 27709 USA NIEHSRes Triangle Pk NC USA 27709 ab, NIH, Res Triangle Pk, NC 27709 USA
Titolo Testata:
BIOLOGY OF REPRODUCTION
fascicolo: 6, volume: 59, anno: 1998,
pagine: 1522 - 1529
SICI:
0006-3363(199812)59:6<1522:EOJSFT>2.0.ZU;2-S
Fonte:
ISI
Lingua:
ENG
Soggetto:
TYROSINE PROTEIN-KINASE; AMINO-TERMINAL DOMAIN; FACTOR RECEPTOR EGFR; TGF-ALPHA; PERIIMPLANTATION PERIOD; CYTOPLASMIC DOMAINS; MENSTRUAL-CYCLE; MOUSE UTERUS; EXPRESSION; TRANSMEMBRANE;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
36
Recensione:
Indirizzi per estratti:
Indirizzo: Bush, MR Madigan Army Med Ctr, MCHG OG, Div Reprod Endocrinol & Infertil, Tacoma, WA Madigan Army Med Ctr Tacoma WA USA 98431 & Infertil, Tacoma, WA
Citazione:
M.R. Bush et al., "Evidence of juxtacrine signaling for transforming growth factor alpha in human endometrium", BIOL REPROD, 59(6), 1998, pp. 1522-1529

Abstract

0To determine the mechanism of signaling for transforming growth factor alpha (TGF alpha) in human endometrium, uterine luminal fluid proteins were retrieved by ravage followed by collection of the adjacent endometrium at hysterectomy, In the endometrium we observed the presence of the full-length transmembrane TGF alpha protein and the phosphorylation of its only known receptor, the epidermal growth factor receptor (EGFR), by immunoprecipitation-Western blot; TCF alpha mRNA via reverse transcription-polymerase chain reaction; and immunolocalization of TCF alpha to the surface endometrium adjacent to the uterine lumen. Despite this demonstration of TGF alpha: in functional endometrium, we could not detect measurable amounts of TGF alpha inany of the 16 endometrial washings by either immunoprecipitation-Western blot or by ELISA. Recovery rate for intraluminal fluid spiked with TGF alphacontrol peptide was 93.4-97%, The inability to detect TGF alpha in intraluminal fluid despite its high concentration in cells directly adjacent to the uterine lumen, along with the absence of any cleaved TGF alpha species identified in the endometrium, suggests that TCF alpha signals its receptor as a transmembrane ligand, Since the EGFR is present in the endometrium and on the surface of embryos, these data are consistent with a juxtacrine modeof signaling for TGF alpha between endometrial cells, and between the luminal surface epithelium and preimplantation embryos.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 31/03/20 alle ore 16:21:04