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Titolo:
Cyclodextrin removes cholesterol from mouse sperm and induces capacitationin a protein-free medium
Autore:
Choi, YH; Toyoda, Y;
Indirizzi:
ObihirooUniv Agr & Vet Med, Res Ctr Protozoan Mol Immunol, Obihiro, Hokkaid Obihiro Univ Agr & Vet Med Obihiro Hokkaido Japan 0808555 bihiro, Hokkaid
Titolo Testata:
BIOLOGY OF REPRODUCTION
fascicolo: 6, volume: 59, anno: 1998,
pagine: 1328 - 1333
SICI:
0006-3363(199812)59:6<1328:CRCFMS>2.0.ZU;2-M
Fonte:
ISI
Lingua:
ENG
Soggetto:
LIGAND-RECEPTOR EXPRESSION; ALBUMIN-MEDIATED CHANGES; IN-VITRO FERTILIZATION; BETA-CYCLODEXTRIN; ACROSOME REACTION; HAMSTER SPERMATOZOA; PLASMA-MEMBRANE; CHLORTETRACYCLINE FLUORESCENCE; PREIMPLANTATION EMBRYOS; INVITRO CAPACITATION;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
56
Recensione:
Indirizzi per estratti:
Indirizzo: Choi, YH Colorado State Univ, Anim Reprod & Biotechnol Lab, Ft Collins, CO80523 USA Colorado State Univ Ft Collins CO USA 80523 ollins, CO 80523 USA
Citazione:
Y.H. Choi e Y. Toyoda, "Cyclodextrin removes cholesterol from mouse sperm and induces capacitationin a protein-free medium", BIOL REPROD, 59(6), 1998, pp. 1328-1333

Abstract

Cyclodextrin, which stimulates cholesterol efflux from cells, was examinedfor its ability to induce capacitation of mouse spermatozoa. A chemically defined, protein-free medium was used for in vitro fertilization of cumulus-free mouse eggs. Fertilization did not occur in modified Krebs-Ringer bicarbonate medium (TYH) supplemented with 1 mg/ml polyvinylalcohol instead of BSA. However, fertilization was observed when spermatozoa were preincubatedwith methyl-beta-cyclodextrin (MBCD); fertilization rates increased dose-dependently from 0.25 to 0.75 mM MBCD, The fertilization rate decreased when0.75 mM MBCD was added to both preincubation and fertilization media versus only the preincubation medium (21% vs. 53%); in sharp contrast, fertilization increased when 4 mg/ml BSA was present in both of the media versus thepreincubation medium only (66% vs. 25%). At 0.75 mM, 2-hydroxy-beta-cyclodextrin had a lower ability to capacitate spermatozoa in vitro than MBCD (14% vs. 41%), Eggs fertilized by spermatozoa treated with MBCD (0.75 mM) developed to blastocysts (45%, 36 of 80) when cultured in KSOM, When 160 fertilized eggs were transferred to ICR recipients, 62 live offspring were born. After incubation of mouse spermatozoa for 90 min in 0.75 mM MBCD in TYH medium, the cholesterol content of the spermatozoa was significantly (p < 0.01) lower than that of the control (2.27 +/- 0.09 vs. 4.13 +/- 0.09 nmol unesterified cholesterol/10(7) sperm; mean +/- SEM, n = 5), The proportion of capacitated (B pattern) spermatozoa determined by chlortetracycline fluorescence was higher with MBCD treatment for 90 min than for the control (45% vs. 15%; p < 0.01), The proportion of acrosome-reacted (AR pattern) spermatozoa was not different between MBCD treatment and the control. Therefore, MBCD increased capacitation rather than the acrosome reaction of spermatozoa.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 29/11/20 alle ore 03:33:27