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Titolo:
Expression of thrombomodulin in atherosclerotic lesions and mitogenic activity of recombinant thrombomodulin in vascular smooth muscle cells
Autore:
Tohda, G; Oida, K; Okada, Y; Kosaka, S; Okada, E; Takahashi, S; Ishii, H; Miyamori, I;
Indirizzi:
Fukui Med Univ, Fac Med, Dept Internal Med 3, Matsuoka, Fukui 91011, JapanFukui Med Univ Matsuoka Fukui Japan 91011 3, Matsuoka, Fukui 91011, Japan Osaka Med Coll, Dept Pathol, Osaka, Japan Osaka Med Coll Osaka JapanOsaka Med Coll, Dept Pathol, Osaka, Japan Showa Coll Pharmaceut Sci, Dept Publ Hlth, Tokyo, Japan Showa Coll Pharmaceut Sci Tokyo Japan Sci, Dept Publ Hlth, Tokyo, Japan
Titolo Testata:
ARTERIOSCLEROSIS THROMBOSIS AND VASCULAR BIOLOGY
fascicolo: 12, volume: 18, anno: 1998,
pagine: 1861 - 1869
SICI:
1079-5642(199812)18:12<1861:EOTIAL>2.0.ZU;2-5
Fonte:
ISI
Lingua:
ENG
Soggetto:
EPIDERMAL GROWTH-FACTOR; PROTEIN-C ACTIVATION; TYROSINE KINASE-ACTIVITY; LOW-DENSITY-LIPOPROTEIN; PLASMA THROMBOMODULIN; SOLUBLE THROMBOMODULIN; ANTICOAGULANT ACTIVITY; MONOCLONAL-ANTIBODIES; ENDOTHELIAL-CELLS; COFACTOR ACTIVITY;
Keywords:
atherosclerosis; thrombomodulin; smooth muscle cells;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
45
Recensione:
Indirizzi per estratti:
Indirizzo: Oida, K Fukui Med Univ, Fac Med, Dept Internal Med 3, Matsuoka, Fukui 91011, Japan Fukui Med Univ Matsuoka Fukui Japan 91011 oka, Fukui 91011, Japan
Citazione:
G. Tohda et al., "Expression of thrombomodulin in atherosclerotic lesions and mitogenic activity of recombinant thrombomodulin in vascular smooth muscle cells", ART THROM V, 18(12), 1998, pp. 1861-1869

Abstract

Thrombomodulin (TM), a thrombin receptor protein found on the endothelial cell surface, contains 6 tandem epidermal growth factor (EGF)-like structures. Recombinant human TM peptide containing these 6 EGF-like domains (rTME1-6) exhibits mitogenic activity in Swiss 3T3 cells. We examined the localization of TM in atherosclerotic lesions and the effects of rTME1-6 on the growth of cultured rat vascular smooth muscle cells (SMCs). Immunohistochemical analysis demonstrated that TM antigen was localized on monocytes, macrophages, and vascular SMCs. In cultured vascular SMCs, rTME1-6 accelerated [H-3]thymidine uptake into DNA in a dose-dependent manner up to 3.4 times thecontrol level. This mitogenic activity was abolished by addition of polyclonal anti-human TM antibody. The rTME1-6-induced mitogenesis was enhanced by EGF. However, a neutralizing monoclonal antibody against the EGF receptor(monoclonal antibody 225) did not inhibit the mitogenic activity of rTME1-6. Calphostin C, a specific protein kinase C inhibitor, and lavendustin-A, an inhibitor of EGF receptor-specific protein tyrosine kinase-inhibited the. mitogenic activities of both rTME1-6 and EGF. Finally, rTME1-6 treatment increased the level of phosphorylated mitogen-activated protein kinase in SMCs. Together, these results suggest that TM expression in atherosclerotic lesions may be associated with promotion of atherosclerosis through its mitogenic activity in vascular SMCs.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 01/12/20 alle ore 10:41:33