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Titolo:
Use of C-13 nuclear magnetic resonance and gas chromatography to examine methionine catabolism by lactococci
Autore:
Gao, S; Mooberry, ES; Steele, JL;
Indirizzi:
Univ Wisconsin, Dept Food Sci, Madison, WI 53706 USA Univ Wisconsin Madison WI USA 53706 Dept Food Sci, Madison, WI 53706 USA Univ Wisconsin, Dept Biochem, Madison, WI 53706 USA Univ Wisconsin Madison WI USA 53706 , Dept Biochem, Madison, WI 53706 USA
Titolo Testata:
APPLIED AND ENVIRONMENTAL MICROBIOLOGY
fascicolo: 12, volume: 64, anno: 1998,
pagine: 4670 - 4675
SICI:
0099-2240(199812)64:12<4670:UOCNMR>2.0.ZU;2-T
Fonte:
ISI
Lingua:
ENG
Soggetto:
LACTIC-ACID BACTERIA; FLAVOR COMPOUND FORMATION; CYSTATHIONINE BETA-LYASE; CHEDDAR CHEESE; DAIRY-PRODUCTS; AMINO-ACIDS; GAMMA-LYASE; METHANETHIOL; MILK; PURIFICATION;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Agriculture,Biology & Environmental Sciences
Life Sciences
Citazioni:
29
Recensione:
Indirizzi per estratti:
Indirizzo: Steele, JL Univ Wisconsin, Dept Food Sci, 1605 Linden Dr, Madison, WI 53706 USA Univ Wisconsin 1605 Linden Dr Madison WI USA 53706 WI 53706 USA
Citazione:
S. Gao et al., "Use of C-13 nuclear magnetic resonance and gas chromatography to examine methionine catabolism by lactococci", APPL ENVIR, 64(12), 1998, pp. 4670-4675

Abstract

Formation of methanethiol from methionine is widely believed to play a significant role in development of cheddar cheese flavor. However, the catabolism of methionine by cheese-related microorganisms has not been well characterized, Two independent methionine catabolic pathways are believed to be present in lactococci, one initiated by a lyase and the other initiated by an aminotransferase. To differentiate between these two pathways and to determine the possible distribution between the pathways, C-13 nuclear magneticresonance (NMR) performed with uniformly enriched [C-13]methionine was utilized, The catabolism of methionine by whole cells and cell extracts of five strains of Lactococcus lactis aas examined, Only the aminotransferase-initiated pathway was observed. The intermediate and major end products were determined to be 4-methylthio-2-oxobutyric acid and acid and 2-hydroxyl-4-methylthiobutyric acid, respectively, Production of methanethiol was not observed in any of the C-13 NMR studies, Gas chromatography was utilized to determine if the products of methionine catabolism in the aminotransferase pathway mere precursors of methanethiol, The results suggest that the direct precursor of methanethiol is 4-methylthiol-2-oxobutyric acid. These results support the conclusion that an aminotransferase initiates the catabolism ofmethionine to methanethiol in lactococci.

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Documento generato il 05/12/20 alle ore 06:31:39