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Titolo:
PCR amplification of Paramecium DNA using the beta-adrenergic-specific primers
Autore:
Wiejak, J; Platek, A; Surmacz, L; Wyroba, E;
Indirizzi:
M Nencki Inst Expt Biol, Dept Cell Biol, PL-02093 Warsaw, Poland M Nencki Inst Expt Biol Warsaw Poland PL-02093 , PL-02093 Warsaw, Poland
Titolo Testata:
ACTA PROTOZOOLOGICA
fascicolo: 4, volume: 37, anno: 1998,
pagine: 215 - 219
SICI:
0065-1583(199811)37:4<215:PAOPDU>2.0.ZU;2-R
Fonte:
ISI
Lingua:
ENG
Soggetto:
BETA-2-ADRENERGIC RECEPTOR; PROTEIN-KINASE; IDENTIFICATION; PHAGOCYTOSIS; GENE; PHOSPHOGLYCOPROTEIN; PHOSPHORYLATION; LOCALIZATION; STIMULATION; RHODOPSIN;
Keywords:
beta-adrenergic receptor; digoxigenin-labeled oligonucleotides DNA; DNA; nucleic acid hybridization; Paramecium aurelia; PCR;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Agriculture,Biology & Environmental Sciences
Citazioni:
29
Recensione:
Indirizzi per estratti:
Indirizzo: Wyroba, E Mlandcki Inst Expt Biol, Dept Cell Biol, Ul Pasteura 3, PL-02093Warsaw, Po M Nencki Inst Expt Biol Ul Pasteura 3 Warsaw Poland PL-02093 Po
Citazione:
J. Wiejak et al., "PCR amplification of Paramecium DNA using the beta-adrenergic-specific primers", ACT PROTOZ, 37(4), 1998, pp. 215-219

Abstract

In continuation to our. previous cytophysiological studies on the effect of beta-adrenergic ligands on Paramecium we present here a new evidence based on molecular investigation of isolated DNA. Specific oligonucleotides designed to hydrophobic transmembrane regions TM 3 and TM 6 of the beta(2)-adrenergic receptor have been applied for hybridization analysis and PCR. In PCR analysis the DNA species of similar to 530 bp were generated which subsequently hybridized to another beta-adrenergic-specific molecular probe located within the amplified region suggesting that the amplified region comprises one of the essential sequence motifs of the beta(2)-adrenergic receptor.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 19/09/20 alle ore 09:29:03