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Titolo:
A regional gradient of cardiac intrinsic rhythmicity depicted in embryoniccultured multiple hearts
Autore:
Sakai, T; Yada, T; Hirota, A; Komura, H; Kamino, K;
Indirizzi:
Tokyoaned & Dent Univ, Sch Med, Dept Physiol, Bunkyo Ku, Tokyo 1138519, Jap Tokyo Med & Dent Univ Tokyo Japan 1138519 Bunkyo Ku, Tokyo 1138519, Jap
Titolo Testata:
PFLUGERS ARCHIV-EUROPEAN JOURNAL OF PHYSIOLOGY
fascicolo: 1, volume: 437, anno: 1998,
pagine: 61 - 69
SICI:
0031-6768(199812)437:1<61:ARGOCI>2.0.ZU;2-3
Fonte:
ISI
Lingua:
ENG
Soggetto:
PRECONTRACTILE CHICK HEART; POTENTIAL-SENSITIVE DYE; ELECTRICAL-ACTIVITY; PATTERNS;
Keywords:
embryonic heart; cultured multiple heart; intrinsic rhythmicity; optical recording; voltage-sensitive dye; cardiac pacemaker; regional gradient; whole-embryo culture;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
30
Recensione:
Indirizzi per estratti:
Indirizzo: Sakai, T Tokyoaned & Dent Univ, Sch Med, Dept Physiol, Bunkyo Ku, Tokyo 1138519, Jap Tokyo Med & Dent Univ Tokyo Japan 1138519 u, Tokyo 1138519, Jap
Citazione:
T. Sakai et al., "A regional gradient of cardiac intrinsic rhythmicity depicted in embryoniccultured multiple hearts", PFLUG ARCH, 437(1), 1998, pp. 61-69

Abstract

We used optical methods to examine the spatial gradient of intrinsic rhythmicity in early-stage multiple-heart chick embryos. The latter were inducedexperimentally in whole-embryo culture. The embryos were cut microsurgically through the tissue of the anterior intestinal portal at the 5- to early 7-somite developmental stage. Spontaneous electrical activity in 4 to 6 segmented hearts, during the 7- to 10-somite stages of development, were monitored simultaneously by means of multiple-site optical recordings of membrane potential activity, using a voltage-sensitive merocyanine-rhodanine dye (NK2761). Each segment of the heart exhibited its own inherent rhythmicity. In quadruple hearts, the order of the rhythmicity was often left-caudal segment>right-caudal segment>left-cephalic segment>right-cephalic segment; theheart rate in the left-caudal segment was often faster than that in the other segments. An atypical pattern of "bursting" rhythm was observed in the cephalic segments suggesting that, in these segments, the development of rhythmicity is relatively poor. These findings strongly emphasize the conceptthat, in the early phases of cardiogenesis, the'formation of a regional gradient of pacemaker activity (i.e. a spatial gradient of intrinsic rhythmicity) results in the functional self-organization of the pacemaking area.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 05/06/20 alle ore 15:59:00