Catalogo Articoli (Spogli Riviste)

OPAC HELP

Titolo:
COMPARATIVE RESPONSE TO UV IRRADIATION OF CYTOSKELETAL ELEMENTS IN RABBIT AND SKATE LENS EPITHELIAL-CELLS
Autore:
RAFFERTY NS; RAFFERTY KA; ZIGMAN S;
Indirizzi:
MARINE BIOL LAB WOODS HOLE MA 02543 UNIV ROCHESTER,SCH MED,OPHTHALMOL RES LAB ROCHESTER NY 00000
Titolo Testata:
Current eye research
fascicolo: 4, volume: 16, anno: 1997,
pagine: 310 - 319
SICI:
0271-3683(1997)16:4<310:CRTUIO>2.0.ZU;2-Z
Fonte:
ISI
Lingua:
ENG
Soggetto:
KERATIN INTERMEDIATE FILAMENTS; CULTURED HUMAN KERATINOCYTES; ULTRAVIOLET-RADIATION; CYTOPLASMIC MICROTUBULES; LIVING CELLS; DNA-DAMAGE; ACTIN; LIGHT; MECHANISM; DYNAMICS;
Keywords:
ACTIN; CYTOSKELETON; LENS; MICROTUBULES; UVA IRRADIATION; VIMENTIN; RABBIT; SKATE;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
42
Recensione:
Indirizzi per estratti:
Citazione:
N.S. Rafferty et al., "COMPARATIVE RESPONSE TO UV IRRADIATION OF CYTOSKELETAL ELEMENTS IN RABBIT AND SKATE LENS EPITHELIAL-CELLS", Current eye research, 16(4), 1997, pp. 310-319

Abstract

Purpose. This work reports a differential effect of ultraviolet A (UVA) irradiation on the three major cytoskeletal structures, actin and vimentin filaments and microtubules of lens cells in primary culture. The effect on cells from lens of the skate (a bottom-dwelling marine elasmobranch) was compared with that on rabbit lens, in order to assess UVA sensitivity as a function of exposure to these wavelengths in the native habitat. Methods. Exposure intervals of irradiation time up to 6 hours were selected, at fluences from 13.5 to 54.4 J/cm(2) and at 365 +/- 45 nm wavelength, to represent mild to moderate physiological levels. Cultures were fixed and processed with anti-alpha-tubulin-FITC and rhodamine phalloidin, or with anti-vimentin FITC and rhodamine phalloidin conjugates. Results. With epifluorescence microscopy, it was found that microtubules were most sensitive to UVA irradiation (in depolymerizing), followed by actin, with vimentin hardly at all affected. Irradiation for 6 hours followed by incubation for 3 days in fresh medium showed no recovery of actin but good recovery of microtubule organizing centers, followed by mitosis in many (rabbit) cells. Skate cells were more sensitive and showed no recovery. Conclusions. In view of the role of cytoskeletal elements in intracellular structure, cell division and transport, their disruption supports the hypothesis that UVA may damage lens epithelial cells in vivo so as to contribute to cataract formation. In addition, the data suggest that the lenses of animals exposed to sunlight require effective cytoskeletal repair mechanisms to avoid loss of function.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 25/09/20 alle ore 00:36:44