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Titolo:
Direct action of serotonin on prolactin, growth hormone, corticotropin andluteinizing hormone release in cocultures of anterior and posterior pituitary lobes: Autocrine and/or paracrine action of vasoactive intestinal peptide
Autore:
Balsa, JA; Sanchez-Franco, F; Pazos, F; Lara, JI; Lorenzo, MJ; Maldonado, G; Cacicedo, L;
Indirizzi:
Hosp Ramon y Cajal, Serv Endocrinol, E-28034 Madrid, Spain Hosp Ramon y Cajal Madrid Spain E-28034 ndocrinol, E-28034 Madrid, Spain Inst Salud Carlos III, CNIC, Madrid, Spain Inst Salud Carlos III Madrid Spain alud Carlos III, CNIC, Madrid, Spain Fdn Jimenez Diaz, E-28040 Madrid, Spain Fdn Jimenez Diaz Madrid Spain E-28040 imenez Diaz, E-28040 Madrid, Spain
Titolo Testata:
NEUROENDOCRINOLOGY
fascicolo: 5, volume: 68, anno: 1998,
pagine: 326 - 333
SICI:
0028-3835(199811)68:5<326:DAOSOP>2.0.ZU;2-H
Fonte:
ISI
Lingua:
ENG
Soggetto:
MELANOCYTE-STIMULATING HORMONE; CENTRAL NERVOUS-SYSTEM; RAT PITUITARY; INTERMEDIATE LOBE; NEUROINTERMEDIATE LOBE; ACTH RELEASE; GLUCOCORTICOID RECEPTOR; PARS-INTERMEDIA; SECRETION; CELLS;
Keywords:
serotonin; vasoactive intestinal peptide; gonadotropins; corticotropin; growth hormone; prolactin;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
50
Recensione:
Indirizzi per estratti:
Indirizzo: Cacicedo, L Hospd,amon y Cajal, Serv Endocrinol, Carretera Colmenar Km 9, E-28034 Madri Hosp Ramon y Cajal Carretera Colmenar Km 9 Madrid Spain E-28034
Citazione:
J.A. Balsa et al., "Direct action of serotonin on prolactin, growth hormone, corticotropin andluteinizing hormone release in cocultures of anterior and posterior pituitary lobes: Autocrine and/or paracrine action of vasoactive intestinal peptide", NEUROENDOCR, 68(5), 1998, pp. 326-333

Abstract

There is extensive evidence that serotonin (5-HT) is implicated in the neuroendocrine control regulating the secretion of several anterior pituitary hormones. It has also been reported that the posterior pituitary is necessary for prolactin (PRL) response to 5-HT as well as to suckling, in which 5-HT implication has been demonstrated. As we have previously shown that vasoactive intestinal peptide (VIP) mediates through an autocrine or paracrine action the PRL release induced by insulin-like growth factor I, thyrotropin-releasing hormone (TRK) and dopamine withdrawal, the aim of the present work was to determine whether 5-HT has a direct action on pituitary secretionand to study the possible role of pituitary VIP in this situation. Cells from the anterior pituitary lobe (AP) were cultured either alone or togetherwith cells from the posterior pituitary lobe (PP). As melanotropes from PPexpress glucocorticoid receptors in vitro, both AP cultures and coculturesof AP/PP cells were incubated in the presence or absence of corticosterone(0.1 mu g/ml), thus designing four experimental conditions. Then both AP and mixed cultures were incubated with 5-HT (100 nhl) for 20, 45 and 180. The release of PRL, growth hormone (GH), corticotropin (ACTH) and luteinizinghormone (LH) was stimulated by 5-HT, but only in cocultures of AP/PP cellspreincubated with corticosterone, whereas follicle-stimulating hormone andthyroid-stimulating hormone release was not modified. As AP cultures did not show any response to 5-HT, both in the presence or absence of corticosterone, and as melanotropes are the main cellular type present in the PP cultures, we studied the response of a-melanocyte-stimulating hormone (aMSH) to5-HT in PP cells cultured with or without corticosterone. Serotonin did not modify aMSH release either in the absence or the presence of corticosterone. VIP release was also stimulated by 5-HT in the cocultures, and the timeresponse profile was only similar to that of PRL. In order to study whether pituitary VIP is implicated in 5-HT action, cocultures preincubated with corticosterone were incubated in the presence of 5-HT, a VIP-receptor antagonist (VIP-At) or simultaneously with 5-HT plus VIP-At. PRL response to 5-HT was abolished by the simultaneous presence of VIP-At, whereas GH, ACTH and LII response remained unchanged. These data demonstrate that: (1) 5-HT stimulates the secretion of PRL, GH, ACTH, LH and VIP acting directly at pituitary level on PP, probably by releasing an unidentified mediator from melanotropes; (2) glucocorticoids make the response of AP cells to 5-HT possible due to the presence of PP cells in the coculture; (3) PRL response to 5-HT is mediated through an autocrine and/or paracrine action of VIP.

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Documento generato il 20/09/20 alle ore 04:25:20