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Titolo:
Expression of zonula occludens and adherens junctional proteins in human venous and arterial endothelial cells: Role of occludin in endothelial solute barriers
Autore:
Kevil, CG; Okayama, N; Trocha, SD; Kalogeris, TJ; Coe, LL; Specian, RD; Davis, CP; Alexander, JS;
Indirizzi:
Louisiana71130e Univ, Med Ctr, Dept Mol & Cellular Physiol, Shreveport, LALouisiana State Univ Shreveport LA USA 71130 ular Physiol, Shreveport, LA Louisiana State Univ, Med Ctr, Dept Surg, Shreveport, LA 71130 USA Louisiana State Univ Shreveport LA USA 71130 rg, Shreveport, LA 71130 USA
Titolo Testata:
MICROCIRCULATION-LONDON
fascicolo: 2-3, volume: 5, anno: 1998,
pagine: 197 - 210
SICI:
1073-9688(199809)5:2-3<197:EOZOAA>2.0.ZU;2-K
Fonte:
ISI
Lingua:
ENG
Soggetto:
TIGHT JUNCTIONS; EPITHELIAL-CELLS; N-CADHERIN; VASCULAR-PERMEABILITY; MEMBRANE-PROTEIN; IN-VITRO; ADHESION; COMPLEX; ZO-1; RAT;
Keywords:
permeability; junctions; occludin; VE-cadherin; gene regulation;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
45
Recensione:
Indirizzi per estratti:
Indirizzo: Alexander, JS Louisianaway,te Univ, Med Ctr, Dept Mol & Cellular Physiol, 1501 Kings High Louisiana State Univ 1501 Kings Highway Shreveport LA USA 71130
Citazione:
C.G. Kevil et al., "Expression of zonula occludens and adherens junctional proteins in human venous and arterial endothelial cells: Role of occludin in endothelial solute barriers", MICROCIRCUL, 5(2-3), 1998, pp. 197-210

Abstract

Objective: The purpose of this study was to correlate the expression of occludin and VE-cadherin with the solute barrier properties of arterial and venous endothelial monolayers. Methods: Immunofluorescent confocal and traditional microscopy were used to determine junctional protein localization in endothelium in vivo and in vitro respectively, and western and northern analysis used to determine protein and gene expression levels. Permeability of endothelial monolayers was examined under normal, low calcium, and cytochalasin-D treatment conditions. Antisense oligonucleotide experiments for occludin were performed to determine the contribution of occludin to solute barrier. Results: Occludin protein in endothelial monolayers is more concentrated in arterial junctions than in venous junctions both bn vivo and in vitro. Arterial endothelial cells express 18-fold more occludin protein and nine times more occludin mRNA compared to venous endothelial cells. In vivo, both endothelial cells demonstrate VE-cadherin staining; and in vitro, only venous endothelial cells express VE-cadherin protein and mRNA. Occludin antisense experiments suggest that both arterial and venous barrier properties are due to these different amounts of occludin expression. Venous barrier was remarkably sensitive to low extracellular calcium, while arterial barrier was more sensitive to cytochalasin-D. Conclusions: These findings suggest strongly that arterial and venous endothelial barrier reflects the level of expression of different adhesion molecules and that modulation of these proteins? especially occludin? may regulate the level of endothelial solute barrier.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 19/09/20 alle ore 08:00:39