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Titolo:
Anti-C5a monoclonal antibody reduces cardiopulmonary bypass and cardioplegia-induced coronary endothelial dysfunction
Autore:
Tofukuji, M; Stahl, GL; Agah, A; Metais, C; Simons, M; Sellke, FW;
Indirizzi:
Beth02215el Deaconess Med Ctr, Dept Med, Div Cardiothorac Surg, Boston, MABeth Israel Deaconess Med Ctr Boston MA USA 02215 thorac Surg, Boston, MA Beth Israel Deaconess Med Ctr, Dept Med, Dept Surg, Div Cardiovasc, Boston, Beth Israel Deaconess Med Ctr Boston MA USA urg, Div Cardiovasc, Boston, Brighamry,Womens Hosp, Dept Anesthesiol, Ctr Expt Therapeut & Reperfus Inju Brigham & Womens Hosp Boston MA USA 02115 Expt Therapeut & Reperfus Inju Harvardry,iv, Sch Med, Dept Anesthesiol, Ctr Expt Therapeut & Reperfus Inju Harvard Univ Boston MA USA esthesiol, Ctr Expt Therapeut & Reperfus Inju
Titolo Testata:
JOURNAL OF THORACIC AND CARDIOVASCULAR SURGERY
fascicolo: 6, volume: 116, anno: 1998,
pagine: 1060 - 1068
SICI:
0022-5223(199812)116:6<1060:AMARCB>2.0.ZU;2-J
Fonte:
ISI
Lingua:
ENG
Soggetto:
NITRIC-OXIDE SYNTHASE; COMPLEMENT ACTIVATION; NEUTROPHIL ACTIVATION; MYOCARDIAL-ISCHEMIA; MOLECULAR-CLONING; EXPRESSION; C5A; CELLS; INTERLEUKIN-8; RELAXATION;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Clinical Medicine
Life Sciences
Citazioni:
26
Recensione:
Indirizzi per estratti:
Indirizzo: Sellke, FW Bethnasrael Deaconess Med Ctr, Dept Med, Div Cardiothorac Surg,E Campus,Da Beth Israel Deaconess Med Ctr E Campus,Dana 905,330 Brookline Ave Boston MA USA 02215
Citazione:
M. Tofukuji et al., "Anti-C5a monoclonal antibody reduces cardiopulmonary bypass and cardioplegia-induced coronary endothelial dysfunction", J THOR SURG, 116(6), 1998, pp. 1060-1068

Abstract

Because C5a induces tissue injury by activating polymorphonuclear leukocytes, the hypothesis was that inhibition of C5a activity would reduce cardioplegia-related injury Methods: Pigs were placed on cardiopulmonary bypass. The hearts were arrested for 1 hour with hyperkalemic cardioplegia. Pigs were then separated from bypass, and the hearts were reperfused for 2 hours. Anti-porcine C5a monoclonal antibody (1.6 mg/kg, intravenously; n = 6) was administered 20 minutes before the onset of cardiopulmonary bypass. Six pigsreceived saline solution vehicle. Reactivity of coronary arterioles was studied in vitro with videomicroscopy, Microvessels from uninstrumented pigs served as controls for vascular studies. Results: Endothelium-dependent relaxation to adenosine diphosphate (percent relaxation of precontraction) wasreduced after cardioplegic reperfusion (63% +/- 14% vs 77% +/- 10% in control at 10 mu mol/L; P = .01), This impairment in endothelium-dependent relaxation was improved with anti-porcine C5a monoclonal antibody (80% +/- 22%;P = .01 vs saline solution), as was the impaired endothelium-dependent relaxation to clonidine (64% +/- 12% control; 26% +/- 17% saline solution; 55%+/- 24% anti-porcine C5a monoclonal antibody at 10 mu mol/L; P = .01 saline solution vs control or anti-porcine C5a monoclonal antibody), Myeloperoxidase activity was significantly decreased (0.2 +/- 0.2 units/g protein; P =.04) in the anti-porcine C5a monoclonal antibody group compared with 5.2 +/- 2.7 in the saline solution group. CH,, 2 hours after bypass was not statistically different (0.57 +/- 0.41 unit and 0.65 +/- 0.41 unit, respectively) between the anti-porcine C5a monoclonal antibody and saline solution groups. Despite less myocardial polymorphonuclear leukocyte infiltration afterC5a inhibition, maximum rate of rise of left ventricular pressure, percentsegmental shortening, and blood flow through the left anterior descending coronary artery were similar in the anti-porcine C5a monoclonal antibody and saline solution groups. Conclusions: Inhibition of C5a limits neutrophil-mediated impairment of endothelium-dependent relaxation after cardiopulmonary bypass and cardioplegic reperfusion, but it has no effect on short-term myocardial functional preservation.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 03/04/20 alle ore 09:26:30