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Titolo:
Expression of stem cell factor (SCF) and SCF receptor (c-kit) in synovial membrane in arthritis: Correlation with synovial mast cell hyperplasia and inflammation
Autore:
Ceponis, A; Konttinen, YT; Takagi, M; Xu, JW; Sorsa, T; Matucci-Cerinic, M; Santavirta, S; Bankl, HC; Valent, P;
Indirizzi:
Orthopaed Hosp Invalid Fdn, ORTON Res Inst, Helsinki 00281, Finland Orthopaed Hosp Invalid Fdn Helsinki Finland 00281 elsinki 00281, Finland Univ Helsinki, Dept Anat, Inst Biomed, FIN-00014 Helsinki, Finland Univ Helsinki Helsinki Finland FIN-00014 ed, FIN-00014 Helsinki, Finland Univnlandinki, Cent Hosp, Dept Med, Div Rheumat Dis, FIN-00014 Helsinki, Fi Univ Helsinki Helsinki Finland FIN-00014 mat Dis, FIN-00014 Helsinki, Fi Univ Helsinki, Cent Hosp, Dept Orthopaed, FIN-00014 Helsinki, Finland UnivHelsinki Helsinki Finland FIN-00014 ed, FIN-00014 Helsinki, Finland Univ Helsinki, Cent Hosp, Dept Traumatol, FIN-00014 Helsinki, Finland UnivHelsinki Helsinki Finland FIN-00014 ol, FIN-00014 Helsinki, Finland Univ Helsinki, Dept Periodontol, Helsinki, Finland Univ Helsinki Helsinki Finland nki, Dept Periodontol, Helsinki, Finland Tohoku Univ, Grad Sch Med, Tohoku, Japan Tohoku Univ Tohoku JapanTohoku Univ, Grad Sch Med, Tohoku, Japan Univ Florence, Ist Clin Med 4, Florence, Italy Univ Florence Florence Italy Florence, Ist Clin Med 4, Florence, Italy Univ Vienna, Dept Clin Pathol, Div Hematol, A-1010 Vienna, Austria Univ Vienna Vienna Austria A-1010 l, Div Hematol, A-1010 Vienna, Austria Univ Vienna, Dept Internal Med 1, A-1010 Vienna, Austria Univ Vienna Vienna Austria A-1010 Internal Med 1, A-1010 Vienna, Austria
Titolo Testata:
JOURNAL OF RHEUMATOLOGY
fascicolo: 12, volume: 25, anno: 1998,
pagine: 2304 - 2314
SICI:
0315-162X(199812)25:12<2304:EOSCF(>2.0.ZU;2-P
Fonte:
ISI
Lingua:
ENG
Soggetto:
RHEUMATOID-ARTHRITIS; GROWTH-FACTOR; HISTAMINE LEVELS; IN-VITRO; LIGAND; ACTIVATION; JOINT; FLUID; DEGRANULATION; MASTOCYTOSIS;
Keywords:
mast cells; stem cell factor; c-kit; rheumatoid arthritis; synovial membrane;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Clinical Medicine
Life Sciences
Citazioni:
51
Recensione:
Indirizzi per estratti:
Indirizzo: Ceponis, A Orthopaed Hosp Invalid Fdn, ORTON Res Inst, POB 29,Tenholantie 10, Helsinki Orthopaed Hosp Invalid Fdn POB 29,Tenholantie 10 Helsinki Finland 00281
Citazione:
A. Ceponis et al., "Expression of stem cell factor (SCF) and SCF receptor (c-kit) in synovial membrane in arthritis: Correlation with synovial mast cell hyperplasia and inflammation", J RHEUMATOL, 25(12), 1998, pp. 2304-2314

Abstract

Objective, Stem cell factor (SCF), the ligand for the SCF receptor (c-kit)expressed on precursors and mature mast cells (MC), is a major agonist forhuman MC (e.g., SCF induces MC development, chemotaxis, activation, proliferation of MC precursors, mediates MC adhesion, and changes MC releasability). We investigated expression of SCF and c-kit in synovial membrane with particular reference to the mechanism of local MC hyperplasia and inflammation in arthritis. Methods. We conducted single and double labeling immunohistochemistry (ABC, APAAP, indirect immunofluorescence techniques) with antibodies to SCE c-kit, MC tryptase, Ki-67 antigen (marker for proliferating cells), and CD68 (monocyte/macrophage marker). Synovial specimens analyzed were from 31 patients: traumatic arthritis (TrA, n=9), osteoarthritis (OA, n=12), and rheumatoid arthritis (RAI n=10). Control experiments were performed on human lung,skin, and buccal mucosa tissues, on the HMC-1 mast cell line, and isolatedlung MC. Morphometry was performed by computerized image analysis. Results. Synovial c-kit expression was found to be restricted to MC, whereas SCF is detected in synovial lining cells, stromal fibroblasts, monocyte/macrophages, endothelial cells, and in vascular basement membranes. SCF staining was localized to MC as well, but it was not possible to specify whether this represents SCF produced by or bound (via c-kit) to MC. Ln inflamed synovial membranes/areas, SCF was found to be redistributed into the extracellular matrix. Redistribution of SCF was accompanied by degranulation and/or accumulation of c-kit+ MC, the hyperplasia of which correlated positively with histologic inflammation/inflammatory cell densities, but did not :appear to involve MC proliferation in situ. These findings appeared to be common for all the conditions (TrA, OA, RA) studied. Conclusion. In addition to the demonstration/characterization of SCF and c-kit protein expression in human synovium, results of this study suggest the hypothesis that, in arthritis, local mobilization of SCF may play a role in the development of synovial MC hyperplasia without inducing in situ proliferation of MC, and that the synovial SCF/MC c-kit system may contribute to the local nonspecific inflammatory response/arthritic flares in TrA, OA, and RA.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 19/09/20 alle ore 20:59:57