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Titolo:
Transplantation of human fetal pancreas: Fresh vs. cultured fetal islets or ICCS
Autore:
Beattie, GM; Lopez, AD; Otonkoski, T; Hayek, A;
Indirizzi:
Univ37alif San Diego, Sch Med, Islet Res Lab, Dept Pediat, La Jolla, CA 920 Univ Calif San Diego La Jolla CA USA 92037 Dept Pediat, La Jolla, CA 920 Univ Helsinki, Transplantat Lab, FIN-00014 Helsinki, Finland Univ Helsinki Helsinki Finland FIN-00014 ab, FIN-00014 Helsinki, Finland Univ Helsinki, Childrens Hosp, FIN-00014 Helsinki, Finland Univ Helsinki Helsinki Finland FIN-00014 sp, FIN-00014 Helsinki, Finland
Titolo Testata:
JOURNAL OF MOLECULAR MEDICINE-JMM
fascicolo: 1, volume: 77, anno: 1999,
pagine: 70 - 73
SICI:
0946-2716(199901)77:1<70:TOHFPF>2.0.ZU;2-U
Fonte:
ISI
Lingua:
ENG
Soggetto:
INSULIN INDEPENDENCE; CELLS; CRYOPRESERVATION; DIFFERENTIATION; GROWTH; TYPE-1; MICE;
Keywords:
human fetal pancreas; transplantation; differentiation;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Clinical Medicine
Life Sciences
Citazioni:
21
Recensione:
Indirizzi per estratti:
Indirizzo: Beattie, GM Univ Calif San Diego, Sch Med, Islet Res Lab, Dept Pediat, 894Genesee Ave, Univ Calif San Diego 894 Genesee Ave La Jolla CA USA 92037 ve,
Citazione:
G.M. Beattie et al., "Transplantation of human fetal pancreas: Fresh vs. cultured fetal islets or ICCS", J MOL MED-J, 77(1), 1999, pp. 70-73

Abstract

The paucity of human adult islets available for transplantation in IDDM makes the use of human fetal pancreas a potential alternative. Fetal pancreatic endocrine cells grow and differentiate over time when fresh explants or cultured islet-like cell cluster (ICCs) are transplanted under the kidney capsule in athymic nude mice. We have recently developed a procedure to isolate fetal islets, which differ from ICCs in their beta-cell content. This study was undertaken to compare the maturation and growth of grafts from purified Fetal islets, containing mostly beta-cells, to grafts of mostly undifferentiated endocrine cell precursors, cultured as ICCs, and fresh, uncultured tissue. Total insulin content was highest in the fresh tissue pre-transplant while insulin levels fell precipitously during culture as either fetal islets or ICCs. Although 500 fetal islets contained more insulin than 500ICCS before transplantation, the insulin content of the resulting grafts was the same 3 months post-transplantation. The degree of stimulation following glucose challenge was comparable, as was the histological appearance. However 70 mg of fresh tissue was needed to generate the fetal islets while only 30 mg was needed for the ICCs. Grafts of 30 mg fresh tissue also had similar total insulin contents and stimulation following glucose challenge, but, when normalized to DNA there was a significantly higher concentration of insulin in the grafts from ICCs or fetal islets. Moreover there were distinct morphological differences, with fibrous and ductal elements prominentin the grafts from fresh tissue, which were also much larger and more diffuse, with cystic elements evident macroscopically. Quantitative immunohistochemical analysis showed that grafts from cultured tissue were 48.3+/-5% positive for immunoreactive insulin compared with grafts from fresh tissue which were only 13.3+/-1.4% positive for insulin. In conclusion cultured ICCs, a heterogeneous mixture of hormone-containing and undifferentiated endocrine cells, are a preferable source for transplantation than either purifiedfetal islets or uncultured tissue.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 10/07/20 alle ore 15:32:09