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Titolo:
Specificity of antibodies to nitric oxide synthase isoforms in human, guinea pig, rat, and mouse tissues
Autore:
Coers, W; Timens, W; Kempinga, C; Klok, PA; Moshage, H;
Indirizzi:
Univ Groningen Hosp, Dept Pathol, NL-9700 RB Groningen, Netherlands Univ Groningen Hosp Groningen Netherlands NL-9700 RB ningen, Netherlands Univtherlandsn Hosp, Dept Gastroenterol & Hepatol, NL-9700 RB Groningen, Ne Univ Groningen Hosp Groningen Netherlands NL-9700 RB 00 RB Groningen, Ne
Titolo Testata:
JOURNAL OF HISTOCHEMISTRY & CYTOCHEMISTRY
fascicolo: 12, volume: 46, anno: 1998,
pagine: 1385 - 1391
SICI:
0022-1554(199812)46:12<1385:SOATNO>2.0.ZU;2-F
Fonte:
ISI
Lingua:
ENG
Soggetto:
LOCALIZATION; EXPRESSION; CLONING; LUNG; EPITHELIUM; CELLS; NOS;
Keywords:
nitric oxide; NOS isoforms; immunohistology; expression; characterization;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
18
Recensione:
Indirizzi per estratti:
Indirizzo: Timens, W Univandsningen Hosp, Dept Pathol, POB 30-001, NL-9700 RB Groningen, Netherl Univ Groningen Hosp POB 30-001 Groningen Netherlands NL-9700 RB
Citazione:
W. Coers et al., "Specificity of antibodies to nitric oxide synthase isoforms in human, guinea pig, rat, and mouse tissues", J HIST CYTO, 46(12), 1998, pp. 1385-1391

Abstract

Ten commercially available rabbit polyclonal anti-NOS antibodies were tested for their immunohistological applicability in normal human, guinea pig, rat, and mouse organs. Most antibodies reacted as expected and described inthe literature with various tissues of the investigated species. Several antibodies did not react with the expected cell populations in a certain species, or reacted in previously unknown patterns. In addition, different antibodies to the same isoform rarely detected identical cell populations, even within one species. Most of these unexpected immunoreactivities were observed in bronchial epithelial, glomerular epithelial, and vascular smooth muscle cells. These unexpected results usually occurred when the antibodies were tested in other organs or species than that to which they were originally raised. We therefore strongly recommend the use of anti-NOS antibodies only after careful immunohistological and biochemical analysis of their reactivity in the organ and species to be studied.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 04/12/20 alle ore 21:51:12