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Titolo:
Development and population study of an eight-locus short tandem repeat (STR) multiplex system
Autore:
Lins, AM; Micka, KA; Sprecher, CJ; Taylor, JA; Bacher, JW; Rabbach, DR; Bever, RA; Creacy, SD; Schumm, JW;
Indirizzi:
Promega Corp, DNA Typing, Res & Dev, Madison, WI 53711 USA Promega Corp Madison WI USA 53711 yping, Res & Dev, Madison, WI 53711 USA BODE Technol, Sterling, VA 20166 USA BODE Technol Sterling VA USA 20166BODE Technol, Sterling, VA 20166 USA Creacy Enterprises Inc, Greensboro, NC 27410 USA Creacy Enterprises Inc Greensboro NC USA 27410 , Greensboro, NC 27410 USA
Titolo Testata:
JOURNAL OF FORENSIC SCIENCES
fascicolo: 6, volume: 43, anno: 1998,
pagine: 1168 - 1180
SICI:
0022-1198(199811)43:6<1168:DAPSOA>2.0.ZU;2-X
Fonte:
ISI
Lingua:
ENG
Soggetto:
HOMOLOGOUS GENE AMELOGENIN; DNA SEX TEST; ALLELIC LADDER; IDENTIFICATION; AMPLIFICATION; MUTATION;
Keywords:
forensic science; DNA typing; short tandem repeats; multiplex; polymerase chain reaction; CSF1PO; TPOX; TH01; vWA; D16S539; D7S820; D13S317; D5S818; F13A01; FESFPS; F13B; LPL; PowerPlex;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Clinical Medicine
Citazioni:
30
Recensione:
Indirizzi per estratti:
Indirizzo: Schumm, JW Promega1Corp, DNA Typing, Res & Dev, 2800 Woods Hollow Rd, Madison, WI 5371 Promega Corp 2800 Woods Hollow Rd Madison WI USA 53711 WI 5371
Citazione:
A.M. Lins et al., "Development and population study of an eight-locus short tandem repeat (STR) multiplex system", J FOREN SCI, 43(6), 1998, pp. 1168-1180

Abstract

Amplification of short tandem repeat (STR) loci has become a useful tool for human identification applications. To improve throughput and efficiency for such uses, the polymorphic STR loci CSF1PO, TPOX, TH01, vWA, D16S539, D7S820, D13S317. D5S818, F13A01, FESFPS, F13B, and LPL have bean evaluated, developed, and configured into fluorescently labeled multiplex systems. Eight of these STR loci were combined to generate the PowerPlex(TM) System, a two-color multiplex system that supports rapid, accurate, reliable analysisand designation of alleles. The remaining four loci comprise the FFFL System, a one-color multiplex system. The PowerPlex(TM) System may be evaluatedalternatively as two one-color, four-locus multiplex systems, CTTv Multiplex and GammaSTR(TM) Multiplex. The products of multiplex amplification may be analyzed with a variety of fluorescence detection instruments. Determination of genotypes of over 200 individuals from each of three different population/ethnic groups revealed independence of inheritance of the loci and allowed calculation of matching probability, typical paternity index, and power of exclusion for each multiplex.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 24/11/20 alle ore 22:34:20