Catalogo Articoli (Spogli Riviste)

OPAC HELP

Titolo:
Effects of heparan-like polymers associated with growth factors on osteoblast proliferation and phenotype expression
Autore:
Blanquaert, F; Barritault, D; Caruelle, JP;
Indirizzi:
Univssulair,2, CNRS UPRESA 7053, Lab Rech Croissance Reparat & Regenerat Ti Univ Paris 12 Creteil France F-94010 h Croissance Reparat & Regenerat Ti
Titolo Testata:
JOURNAL OF BIOMEDICAL MATERIALS RESEARCH
fascicolo: 1, volume: 44, anno: 1999,
pagine: 63 - 72
SICI:
0021-9304(199901)44:1<63:EOHPAW>2.0.ZU;2-N
Fonte:
ISI
Lingua:
ENG
Soggetto:
PLASMINOGEN-ACTIVATOR PRODUCTION; DEXTRAN DERIVATIVES; EXTRACELLULAR-MATRIX; AFFINITY-CHROMATOGRAPHY; PROTEOLYTIC DEGRADATION; ANGIOGENIC PROTEIN; CRANIOTOMY DEFECTS; FRACTURE REPAIR; BONE-FORMATION; FIBROBLAST;
Keywords:
osteoblast; heparan-like molecules; heparin-binding growth factors; DNA synthesis; phenotype expression;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
50
Recensione:
Indirizzi per estratti:
Indirizzo: Barritault, D Univssulair,2, CNRS UPRESA 7053, Lab Rech Croissance Reparat& Regenerat Ti Univ Paris 12 Ave Gen de Gaulle Creteil France F-94010 t Ti
Citazione:
F. Blanquaert et al., "Effects of heparan-like polymers associated with growth factors on osteoblast proliferation and phenotype expression", J BIOMED MR, 44(1), 1999, pp. 63-72

Abstract

Heparan-like polymers derived from dextran, named RGTA, were shown to stimulate bone repair in different bone defect models. Like heparin and heparansulfates, RGTA potentiate in vitro the biological activities of heparin-binding growth factors (HBGFs), such as fibroblast growth factor (FGF), by stabilizing them against denaturations and by enhancing their binding with cellular receptors. RGTA were postulated to stimulate bone healing by interacting with HBGFs released in the wound site and, subsequently, by promoting the proliferation and/or differentiation of cells implicated in this process. We examined the effects of RGTA alone and associated with HBGFs on MC3T3-E1 osteoblastic cell proliferation and differentiation. RGTA inhibited cell proliferation, as measured by [H-3]thymidine incorporation into DNA. Theyenhanced the inhibition of DNA synthesis caused by transforming growth factor-beta (TGF-beta 1) and bone morphogenetic protein-2 (BMP-2). RGTA alone increased the alkaline phosphatase and parathyroid hormone-responsive adenylate cyclase activities in MC3T3. RGTA enhanced the stimulation of the alkaline phosphatase activity induced by BMP-2 and decreased or suppressed the inhibition caused by TGF-beta 1 and FGF-2. Furthermore, RGTA increased the response to parathyroid hormone stimulated by BMP-2. In conclusion, RGTA stimulate the expression of osteoblast phenotype features alone or in association with HBGFs. The ability to promote the differentiation of bone-formingcells is a potential explanation of the stimulating effect of RGTA on bonerepair. (C) 1999 John Wiley & Sons, Inc.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 01/12/20 alle ore 11:00:14