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Titolo:
GATA6 regulates HNF4 and is required for differentiation of visceral endoderm in the mouse embryo
Autore:
Morrisey, EE; Tang, ZH; Sigrist, K; Lu, MM; Jiang, F; Ip, HS; Parmacek, MS;
Indirizzi:
Univ Chicago, Dept Med, Chicago, IL 60637 USA Univ Chicago Chicago IL USA60637 hicago, Dept Med, Chicago, IL 60637 USA Univ Penn, Dept Med, Philadelphia, PA 19104 USA Univ Penn Philadelphia PAUSA 19104 Dept Med, Philadelphia, PA 19104 USA
Titolo Testata:
GENES & DEVELOPMENT
fascicolo: 22, volume: 12, anno: 1998,
pagine: 3579 - 3590
SICI:
0890-9369(19981115)12:22<3579:GRHAIR>2.0.ZU;2-#
Fonte:
ISI
Lingua:
ENG
Soggetto:
TRANSCRIPTION FACTOR HNF-4; HEART TUBE FORMATION; CAENORHABDITIS-ELEGANS; GENE-EXPRESSION; BINDING PROTEIN; VENTRAL MORPHOGENESIS; ENDOTHELIAL-CELLS; HOMEOBOX GENE; C GENE; PROMOTER;
Keywords:
endoderm; transcription factor; HNF4; GATA;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
50
Recensione:
Indirizzi per estratti:
Indirizzo: Parmacek, MS Univ Chicago, Dept Med, 5841 S Maryland Ave, Chicago, IL 60637 USA Univ Chicago 5841 S Maryland Ave Chicago IL USA 60637 637 USA
Citazione:
E.E. Morrisey et al., "GATA6 regulates HNF4 and is required for differentiation of visceral endoderm in the mouse embryo", GENE DEV, 12(22), 1998, pp. 3579-3590

Abstract

GATA6 belongs to a family of zinc finger transcription factors that play important roles in transducing nuclear events that regulate cellular differentiation and embryonic morphogenesis in vertebrate species. To examine the function of GATA6 during embryonic development, gene targeting was used to generate GATA6-deficient (GATA6(-/-)) ES cells and mice harboring a null mutation in GATA6. Differentiated embryoid bodies derived from GATA6(-/-) ES cells lack a covering layer of visceral endoderm and severely attenuate, orfail to express, genes encoding early and late endodermal markers, including HNF4, GATA4, rw-fetoprotein (AFP), and HNF3 beta. Homozygous GATA6(-/-) mice died between embryonic day (E) 6.5 and E7.5 and exhibited a specific defect in endoderm differentiation including severely down-regulated expression of GATA4 and absence of HNF4 gene expression. Moreover, widespread programmed cell death was observed within the embryonic ectoderm of GATA6-deficient embryos, a finding also observed in HNF4-deficient embryos. Consistentwith these data, forced expression of GATA6 activated the HNF4 promoter innonendodermal cells. Finally, to examine the function of GATA6 during later embryonic development, GATA6(-/-)-C57BL/6 chimeric mice were generated, lacZ-tagged GATA6(-/-) ES cells contributed to all embryonic tissues with the exception of the endodermally derived bronchial epithelium. Taken together, these data suggest a model in which GATA6 lies upstream of HNF4 in a transcriptional cascade that regulates differentiation of the visceral endoderm. In addition, these data demonstrate that GATA6 is required for establishment of the endodermally derived bronchial epithelium.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 25/09/20 alle ore 13:32:05